Nucleic acids encoding human calcium channel and methods of use thereof
OwnerMichael M. Harpold, Steven B. Ellis +1inventors
InventorsMichael M. Harpold, Steven B. Ellis, Mark E. Williams
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Abstract
Isolated DNA encoding each of human calcium channel α1-, α2-, β- and γ-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.
Claims68
An isolated nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule that encodes an α1E-subunit of a human calcium channel and comprises the coding portion of the sequence of nucleotides set forth in SEQ ID NO:
- 24;
- (b) a nucleic acid molecule that encodes an α1E subunit of a human calcium channel and comprises the coding portion of the sequence of nucleotides set forth in SEQ ID NO:
- 27;
- (c) a nucleic acid molecule that encodes an α1E subunit of a human calcium channel and comprises the sequence of nucleotides set forth in SEQ ID NO:
- 25, wherein the encoded α1E subunit has a molecular weight greater than about 120 kilodaltons (kD) and is a full-length α1E subunit of a human calcium channel that can form an ion channel;
- (d) a nucleic acid molecule comprising a sequence of nucleotides with codons that are degenerate to the codons in the coding portion of the sequence of nucleotides set forth in (a) or (b) above;
- (e) a nucleic acid molecule comprising a sequence of nucleotides that encodes an α1E subunit of a human calcium channel that comprises a sequence of amino acid sequence encoded by the nucleic acid molecule of any one of (a), (b), (c) or (d) above;
- and
- (f) a nucleic acid molecule that encodes an α1E subunit of a human calcium channel polypeptide, wherein the nucleic acid molecule hybridizes under stringent wash condition, 0.1.times.SSC, 0.1% SDS at 65° C., to the complement of the nucleotide sequence of (a), (b), (c), (d) or (e) above.
An isolated nucleic acid molecule, comprising a sequence of amino acids encoded by the sequence of nucleotides set forth in SEQ ID NO. 24.
An isolated nucleic acid molecule, comprising a sequence of amino acids encoded by the sequence of nucleotides set forth in SEQ ID NO. 27.
An isolated nucleic acid molecule, comprising the sequence of nucleotides set forth in nucleotides 169-6921 of SEQ ID No. 24.
An isolated nucleic acid molecule that encodes an α1E subunit of a human calcium channel and comprises a sequence of amino acids encoded by the sequence of nucleotides set forth in nucleotides 166-6978 of SEQ ID NO. 27.
An isolated nucleic acid molecule, comprising the sequence of nucleotides set forth in nucleotides 166-6978 of SEQ ID NO. 27.
An isolated nucleic acid molecule that encodes an α1E-subunit of a human calcium channel comprising a sequence of amino acids encoded by the sequence of nucleotides set forth in nucleotides 166-6978 of SEQ ID NO. 27.
An isolated RNA molecule selected from the group consisting of:
- (a) a nucleic acid molecule that encodes an α1E-subunit of a human calcium channel and comprises the coding portion of the sequence of nucleotides set forth in SEQ ID NO:
- 24;
- (b) a nucleic acid molecule that encodes an α1E subunit of a human calcium channel and comprises the coding portion of the sequence of nucleotides set forth in SEQ ID NO:
- 27;
- (c) a nucleic acid molecule that encodes an α1E subunit of a human calcium channel and comprises the sequence of nucleotides set forth in SEQ ID NO:
- 25, wherein the encoded α1E subunit has a molecular weight greater than about 120 kilodaltons (kD) and is a full-length α1E subunit of a human calcium channel that can form an ion channel;
- (d) a nucleic acid molecule comprising a sequence of nucleotides with codons that are degenerate to the codons in the coding portion of the sequence of nucleotides set forth in (a) or (b) above;
- (e) a nucleic acid molecule comprising a sequence of nucleotides that encodes an α1E subunit of a human calcium channel that comprises a sequence of amino acid sequence encoded by the nucleic acid molecule of any one of (a), (b), (c) or (d) above;
- and
- (f) a nucleic acid molecule that encodes an α1E subunit of a human calcium channel polypeptide, wherein the nucleic acid molecule hybridizes under stringent wash condition, 0.1.times.SSC, 0.1% SDS at 65° C., to the complement of the nucleotide sequence of (a), (b), (c), (d) or (e) above.
An isolated nucleic acid molecule that encodes an α1E-subunit of a human calcium channel, wherein the α1E-subunit can form a functional calcium channel.
Dependencies are inferred from the claim text.