System for producing synthetic promoters
AssigneePromogen, Inc.
InventorSung Ho Hahm
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Abstract
The present application discloses a method for making and selecting a transcription enhancing combined promoter cassette, which includes constructing a library of randomly combined transcription regulatory elements, which comprise double stranded DNA sequence elements that are recognized by transcription regulators; inserting the combined transcription regulatory elements upstream of a minimum promoter followed by a reporter gene in a vector; inserting the vector into a host cell; and then screening for the cells showing enhanced expression of the reporter gene, and identifying the combined promoter cassette in the cell.
Claims12
A method for making and selecting a transcription enhancing combined promoter cassette comprising:
- (i) constructing a library of randomly combined transcription regulatory elements, each of which comprises double stranded DNA sequence elements that are recognized by a specific transcription regulator and flanking sequences;
- (ii) inserting the combined transcription regulatory elements upstream of a minimum promoter followed by a reporter gene in a vector;
- (iii) inserting the vector into a host cell;
- and
- (iv) screening for the cells showing enhanced expression of the reporter gene, and identifying the combined promoter cassette in the cell,
- wherein the DNA sequence elements that are recognized by transcription regulators are obtained by DNA microarray profiling of transcription regulators and co-regulators.
A method for making and selecting a transcription enhancing combined promoter cassette comprising:
- (i) constructing a library of randomly combined transcription regulatory elements, each of which comprises double stranded DNA sequence elements that are recognized by a specific transcription regulator and flanking sequences;
- (ii) inserting the combined transcription regulatory elements upstream of a minimum promoter followed by a reporter gene in a vector;
- (iii) inserting the vector into a neuroblastoma host cell;
- and
- (iv) screening for the cells showing enhanced expression of the reporter gene, and identifying the combined promoter cassette in the cell.
Dependencies are inferred from the claim text.