DNA immunization against Chlamydia infection
AssigneeUniversity of Manitoba
InventorRobert C. Brunham
Life of this patent
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Abstract
Nucleic acid, including DNA, for immunization to generate a protective immune response in a host, including humans, to a major outer membrane protein of a strain of Chlamydia, preferably contains a nucleotide sequence encoding a fragment that generates antibodies that specifically react with MOMP and a promoter sequence operatively coupled to the first nucleotide sequence for expression of the MOMP fragment in the host. The non-replicating vector may be formulated with a pharmaceutically-acceptable carrier for in vivo administration to the host.
Claims25
A method of immunizing a host against disease caused by infection with a strain of Chlamydia, which comprises administering to said host an effective amount of a non-replicating vector comprising:
- a nucleotide sequence encoding a region consisting of at least one of the conserved domains 2, 3 and 5 of a major outer membrane protein (MOMP) of a strain of Chlamydia, and
- a promoter sequence operatively coupled to said nucleotide sequence for expression of said at least one conserved domain in the host.
A method of using a nucleotide sequence encoding a fragment of a major outer membrane protein (MOMP) of a strain of Chiamydia that generates a MOMP-specific immune response, to produce an immune response in a host, which comprises:
- isolating said nucleotide sequence encoding a region consisting of at least one of the conserved domains 2, 3 and 5 of a major outer membrane protein of a strain of Chlamydia,
- operatively linking said nucleotide sequence to at least one control sequence to produce a non-replicating vector, said control sequence directing expression of said MOMP fragment when introduced into a host to produce an immune response to said MOMP fragment, and
- introducing said vector into a host.
A method of using a nucleotide sequence encoding a fragment of a major outer membrane protein (MOMP) of a strain of Chlamydia that generates a MOMP-specific immune response, to produce an immune response in a host, which comprises:
- isolating a nucleotide sequence encoding a region consisting of at least one of the conserved domains 2 and 3 of the MOMP of a strain of Chiamydia and further consisting of a nucleotide sequence encoding a variable domain of the major outer membrane protein immediately downstream of said conserved domain,
- operatively linking said nucleotide sequence to at least one control sequence to produce a non-replicating vector, said control sequence directing expression of said MOMP fragment when introduced into a host to produce an immune response to said MOMP fragment, and
- introducing said vector into a host.
A method of immunizing a host against disease caused by infection with a strain of Chlamydia, which comprises administering to said host an effective amount of a non-replicating vector comprising:
- a nucleotide sequence encoding a region consisting of at least one of the conserved domains 2 and 3 of a major outer membrane protein (MOM P) of a strain of Chlamydia and further consisting of a nucleotide sequence encoding a variable domain of the major outer membrane protein immediately downstream of said conserved domain, and
- a promoter sequence operatively coupled to said nucleotide sequence for expression of said at least one conserved domain and variable domain in the host.
Dependencies are inferred from the claim text.