Electrochemical detection of ischemia
AssigneeIschemia Technologies, Inc.
InventorsHollie Wayment, Gary Fagan, Peter A. Crosby, Shannon George
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Abstract
The subject invention comprises electrochemical methods and devices for in vitro detection of an ischemic event in a patient sample. Following addition of a known amount of a transition metal ion to the patient sample, electrodes are used to measure the current or potential difference of non-sequestered transition metal ion in the sample. The amount of non-sequestered transition metal ion in the sample reflects the degree of modification to albumin that is the result of an ischemic event.
Claims33
A method for in vitro detection or measurement of albumin derivatives, which can be diagnostic of an ischemic event, said method comprising:
- a) providing a patient sample comprising albumin and/or derivatives thereof in a vessel that is connected to an electrochemistry apparatus;
- b) operating the electrochemistry apparatus to utilize an electrochemical technique;
- c) optionally measuring a background characteristic electrochemical output signal of the sample;
- d) adding a known amount of a transition metal ion to the sample, whereby at least some of the ion binds to albumin metal sequestering sites and remaining ion is non-sequestered;
- e) optionally adding an indicator or amplifier to the sample to bind to the non-sequestered ion;
- f) measuring a characteristic electrochemical output signal associated with the non-sequestered ion;
- g) optionally using a standard curve to convert the measured output signal of step (f) to a converted value;
- whereby the measured electrochemical output signal or the converted value, if it exceeds a predetermined value, can be diagnostic of an ischemic event.
A method for in vitro detection or measurement of albumin derivatives, which can be diagnostic for an ischemic event, said method comprising:
- a) providing a patient sample comprising albumin and/or derivatives thereof in a vessel having a reference electrode and a working electrode, said electrodes being operably connected to a potentiostat;
- b) operating the potentiostat to apply a potential to the sample;
- c) optionally offsetting a background current from redox reactions in the sample to zero;
- d) adding a known amount of a transition metal ion to the sample, whereby at least some of the ion binds to albumin metal sequestering sites and remaining ion is non-sequestered by albumin;
- e) optionally providing an indicator or amplifier to the sample to bind to the non-sequestered metal ion;
- f) permitting the reaction of ion and albumin to reach equilibrium or a predetennined processing time;
- and
- g) measuring the current associated with the non-sequestered ion;
- h) optionally using a standard curve to convert the measured current to a converted value;
- whereby the measured current or converted value, if it exceeds a predetermined value, can be diagnostic of an ischemic event.
A method for in vitro detection or measurement of albumin derivatives which may be diagnostic for an ischemic event, said method comprising:
- a) providing a patient sample comprising albumin and/or derivatives thereof in a vessel having an ion selective electrode and a reference electrode, said electrodes being operably linked to an electrostat or a voltmeter, said ion selective electrode comprising a barrier that is selectively permeable to a transition metal ion;
- b) operating the electrostat or voltmeter to measure the potential difference between the reference electrode and the ion selective electrode in contact with the sample;
- c) optionally offsetting a background potential difference of the sample;
- d) adding a known amount of said transition metal ion to the sample, whereby at least some of the ion binds to albumin metal sequestering sites and remaining ion is non-sequestered;
- e) optionally providing an indicator or amplifier to the sample to bind with the non-sequestered metal ion;
- f) permitting the non-sequestered metal ion to cross the membrane of the ion selective electrode;
- and
- g) measuring a voltage associated with an ion activity difference across the ion selective electrode's barrier;
- h) optionally using a standard curve to convert the measured voltage of step (g) to a converted value;
- whereby the measured voltage or the converted value, if it exceeds a predetermined value, can be diagnostic of an ischemic event.
Dependencies are inferred from the claim text.