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US 7,060,431 B2Granted
Method of making and decoding of array sensors with microspheres
AssigneeIllumina, Inc.
InventorsMark S. Chee, John R. Stuelpnagel, Anthony W. Czarnik
Life of this patent
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Abstract
The invention relates to compositions and methods for decoding microsphere array sensors.
Claims76
A method of making a bead array comprising:
- a) contacting a substrate with a surface comprising discrete sites at a density of at least 100 sites per 1 mm2, with a solution comprising a population of different beads, wherein said beads do not comprise an optical signature;
- and
- b) applying energy to said substrate or said solution, or both, such that at least a subpopulation of said different beads randomly associate onto sites.
A method of determining the presence of a target analyte in a sample comprising:
- a) contacting said sample with an array comprising:
- i) substrate with a surface comprising discrete sites at a density of at least 100 sites per 1 mm2, wherein said sites are wells;
- and
- ii) a population of microspheres randomly distributed on said sites, wherein said population comprises at least a first and a second subpopulation each comprising:
- (a) a different bioactive agent;
- and
- (b) a different identifier binding ligand;
- b) determining the presence or absence of said target analyte;
- and
- c) decoding a location of said bioactive agent by correlating said bioactive agent with said location, whereby said decoding comprises contacting said array with at least first and second different decoder binding ligands, whereby said first and second decoder binding ligands bind to a first and second identifier binding ligands, whereby said first and second identifier binding ligands identify said first and second bioactive agents, respectively, to thereby identify a location of said first and second bioactive agents to thereby decode said array.
7 more elements
A method of determining the presence of a target analyte in a sample comprising:
- a) contacting said sample with an array comprising:
- i) a substrate with a surface comprising sites at a density of at least 100 sites per 1 mm2, wherein said sites are present in a depression;
- and
- ii) a population of particles randomly distributed on said sites, wherein said population comprises at least a first and a second subpopulation each comprising:
- (a) a different bioactive agent;
- and
- (b) a different identifier binding ligand;
- b) decoding a location of said bioactive agent by correlating said bioactive agent with said location, whereby said decoding comprises contacting said array with at least first and second different decoder binding ligands, whereby said first and second decoder binding ligands bind to a first and second identifier binding ligands, whereby said first and second identifier binding ligands identify said first and second bioactive agents, respectively, to thereby identify a location of said first and second bioactive agents to thereby decode said array;
- and
- c) determining the presence or absence of said target analyte.
7 more elements
A method of determining the presence of a target analyte in a sample comprising:
- a) contacting said sample with an array comprising:
- i) a substrate with a surface comprising sites at a density of at least 100 sites per 1 mm2, wherein said sites comprise wells;
- and
- ii) a population of particles randomly distributed on said sites, wherein said population comprises at least a first and a second subpopulation each comprising:
- (a) a different bioactive agent;
- and
- (b) a different identifier binding ligand;
- b) decoding a location of said bioactive agent by correlating said bioactive agent with said location, whereby said decoding comprises contacting said array with at least first and second different decoder binding ligands, whereby said first and second decoder binding ligands bind to a first and second identifier binding ligands, whereby said first and second identifier binding ligands identify said first and second bioactive agents, respectively, to thereby identify a location of said first and second bioactive agents to thereby decode said array;
- and
- c) determining the presence or absence of said target analyte.
7 more elements
A method of making a particle array comprising:
- a) contacting a substrate with a surface comprising sites at a density of at least 100 sites per 1 mm2, with a solution comprising a population of different particles, wherein said particles do not comprise an optical signature, and wherein said sites comprise wells;
- and
- b) applying energy to said substrate or said solution, or both, such that at least a subpopulation of said different particles randomly associate onto sites.
Dependencies are inferred from the claim text.