Methods and apparatus for spinning spider silk protein
AssigneeNexia Biotechnologies, Inc.
InventorsShafiul Islam, Costas Karatzas, Andrew Rodenhiser, Ali Alwattari, Yue Huang, Carl Turcotte
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Abstract
The invention features methods and apparatuses for spinning silk protein fibers (biofilaments) from recombinant biofilament proteins. The methods are particularly useful for spinning fibers of spider silk or silkworm silk proteins from recombinant mammalian cells and may be used to spin such fibers for use in the manufacture of industrial and commercial products.
Claims31
A method for producing a spider silk fiber, said method comprising extruding a dope solution comprising a recombinant spider silk protein, through a spinneret to form said spider silk fiber, wherein said recombinant spider silk protein is recovered from mammalian cell culture media, the milk of a transgenic mammal engineered to express said spider silk protein in its milk, the urine of a transgenic mammal, or an extract or exudate from a transgenic plant.
A method for producing a spider silk fiber, said method comprising extruding a dope solution through a spinneret to form said spider silk fiber, wherein said dope solution comprises two or more different spider silk proteins.
The method of claim 24, wherein said spinneret has tube lengths of less than 10 mm.
Dependencies are inferred from the claim text.