Cassava vein mosaic virus promoter nucleic acid sequences and expression vectors
AssigneeThe Scripps Research Institute
InventorsBertrand Verdaguer, Alexandre de Kochko, Roger N. Beachy, Claude Fauguet
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Abstract
The invention relates to compositions useful for the production of transgenic plants. In particular, the invention relates to Cassava vein mosaic virus (CsVMV) promoter sequences and expression cassettes containing CsVMV promoter sequences. The invention describes nucleic acid molecules, vectors and transgenic plants containing promoters derived from CsVMV promoter that are operatively linked to heterologous DNA sequences, and methods for producing transgenic plants containing these promoters.
Claims18
An isolated nucleic acid molecule comprising a promoter nucleotide sequence that initiates transcription of an operably linked heterologous nucleic acid sequence in a plant cell wherein said promoter nucleotide sequence has at least 95% identity to 18 sequential nucleotides of the cassava vein mosaic virus (CsVMV) promoter shown in SEQ ID NO 3 (pA) and wherein said isolated nucleic acid molecule does not include a naturally occurring CsVMV genomic sequence adjacent to said promoter nucleotide sequence.
A vector comprising an isolated promoter nucleotide sequence that is capable of initiating transcription of an operably linked heterologous nucleic acid sequence in a plant cell wherein said promoter nucleotide sequence has at least 95% identity to 18 sequential nucleotides of the cassava vein mosaic virus (CsVMV) promoter shown in SEQ ID NO 3 (pA) and wherein said promoter nucleotide sequence is operatively linked to the heterologous nucleic acid sequence, and wherein said isolated promoter nucleotide sequence does not include a naturally occurring CsVMV genomic sequence adjacent to said promoter nucleotide sequence.
An isolated nucleic acid molecule, comprising:
- a CsVMV promoter nucleotide sequence that initiates transcription of an operably linked heterologous nucleic acid sequence in a plant cell, wherein said isolated nucleic acid molecule does not include a naturally occurring CsVMV genomic sequence adjacent to said CsVMV promoter nucleotide sequence.
An isolated nucleic acid molecule, comprising:
- a CsVMV promoter nucleotide sequence that initiates transcription of an operably linked heterologous nucleic acid sequence in a plant cell, wherein the CsVMV promoter nucleotide sequence comprises SEQ ID NO:3.
An isolated polynucleotide comprising SEQ ID NO:3, or a fragment thereof having promoter activity, wherein said isolated polynucleotide does not include a naturally occurring CsVMV genomic sequence adjacent to said CsVMV promoter nucleotide sequence.
Dependencies are inferred from the claim text.