Back to results
US 7,045,337 B2Granted
In vivo incorporation of unnatural amino acids
AssigneesThe Scripps Research Institute, The Regents of the University of California
InventorsPeter Schultz, Lei Wang, John Christopher Anderson, Jason William Chin, David R. Liu, Thomas J. Magliery, Eric L. Meggers, Ryan A. Mehl, Miro Pastrnak, Steven William Santoro, Zhiwen Zhang
Life of this patent
Estimating the term…
Abstract
The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.
Claims15
A cell comprising:
- (a.) an orthogonal tRNA synthetase that aminoacylates endogenous tRNAs of the cell with a reduced efficiency as compared to aminoacylation of the endogenous tRNAs by endogenous tRNA synthetases of the cell, and
- (b.) an orthogonal tRNA that is aminoacylated by the endogenous tRNA synthetases with reduced efficiency as compared to aminoacylation of the endogenous tRNAs by the endogenous tRNA synthetases,
- wherein said orthogonal tRNA synthetase preferentially aminoacylates said orthogonal tRNA, as compared to the endogenous tRNAs, with an unnatural amino acid in said cell, and
- wherein the orthogonal tRNA synthetase preferentially aminoacylates aid orthogonal tRNA with an unnatural amino acid, as compared to a natural amino acid, the orthogonal tRNA synthetase having a lower Km for the unnatural amino acid than for the natural amino acid, and
- wheein said orthogonal tRNA recognizes a selector codon of an mRNA in said cell.
2 more elements
Dependencies are inferred from the claim text.