Back to results
US 7,045,148 B2Granted
Method of collecting placental stem cells
AssigneeAnthrogenesis Corporation
InventorRobert J. Hariri
Life of this patent
Estimating the term…
Abstract
A method of collecting embryonic-like stem cells from a placenta which has been treated to remove residual cord blood by perfusing the drained placenta with an anticoagulant solution to flush out residual cells, collecting the residual cells and perfusion liquid from the drained placenta, and separating the embryonic-like cells from the residual cells and perfusion liquid. Exogenous cells can be propagated in the placental bioreactor and bioactive molecules collected therefrom.
Claims20
A method of collecting CD34+ stem cells from an isolated and exsanguinated mammalian placenta, said method comprising:
- perfusing said placenta with a perfusion solution in an amount and for a time sufficient to collect a detectable amount of said cells comprising CD34+ stem cells, wherein said placenta has been drained of cord blood and flushed to remove residual blood prior to said perfusing, wherein said CD34+ stem cells are not obtained from cord blood, and wherein said perfusing is performed by passing said perfusion solution into one or both of the umbilical artery and umbilical vein of said placenta;
- and
- collecting said CD34+ stem cells and perfusion solution from said placenta.
Dependencies are inferred from the claim text.