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US 7,041,817 B2Granted
Synthesis, deprotection, analysis and purification of RNA and ribozymes
AssigneeSirna Therapeutics, Inc.
InventorsNassim Usman, Francine Wincott, David Sweedler, Leonid Beigelman, Lech W. Dudycz, Susan Grimm, Anthony DiRenzo, Danuta Tracz
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Abstract
Method for purification and synthesis of RNA molecules and enzymatic RNA molecules in enzymatically active form.
Claims12
A process for deprotecting and purifying chemically synthesized RNA of length between 28 and 70 nucleotides and having one or more chemical modifications, comprising:
- (a) contacting said RNA with an alkylamine under conditions for removing any exocyclic amine protecting groups or phosphate ester protecting groups;
- (b) contacting said RNA with triethylamine-hydrogen fluoride under conditions to remove any alkylsilyl protecting groups from said RNA;
- (c) loading said RNA onto an anion exchange high-performance liquid chromatography (HPLC) column;
- (d) eluting said RNA by passing a buffer through said column;
- and
- (e) collecting the eluate from said column and recovering said RNA from said eluate, under conditions which allow for the purification of said RNA.
3 more elements
Dependencies are inferred from the claim text.