DNA encoding ovine adenovirus (OAV287) and its use as a viral vector
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsGerald Wayne Both, David Bernard Boyle, Sadhanshu Vrati
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Abstract
A genome of an ovine adenovirus designated OAV287 is isolated from sheep and sequenced. Portions of the genome not essential for maintenance or viability of the virus can be deleted or altered. A nucleotide sequence encoding a non-adenoviral polypeptide can be incorporated into the genome. The a full-length clone of the genome can be provided as part of a plasmid or viral vector. Cells can be transformed with a vector of the invention such that they express an exogenous protein.
Claims20
An isolated DNA molecule comprising nucleotides 1–29,574 of SEQ ID NO. 3 or an isolated DNA molecule that hybridizes to the complement of nucleotides 1–29,574 of SEQ ID NO. 3 under high stringency and which is capable of replicating autonomously as an adenovirus in sheep cells.
An isolated DNA molecule comprising the OAV287 inverted terminal repeat consisting of nucleotides 1 through 46 of SEQ ID NO. 3.
An isolated DNA molecule comprising a nucleotide sequence identical to nucleotides 1–29,574 of SEQ ID NO:
- 3 except for a deletion or alteration in all or part of the open reading frame that spans a unique SalI site at nucleotides 28673–28678 of SEQ ID NO:
- 3.
A plasmid comprising a bacterial origin of replication and a first nucleotide sequence as set forth in nucleotides 1–29,574 of SEQ ID NO. 3 or a second nucleotide sequence that specifically hybridizes to the complement of nucleotides 1–29,574 of SEQ ID NO. 3 under high stringency conditions and which is capable of replicating autonomously as an adenovirus in sheep cells.
An adenoviral vector which is capable of replicating autonomously as an adenovirus in sheep cells comprising (1) a first nucleotide sequence having the sequence as set forth in nucleotides 1–29,574 of SEQ ID NO. 3 or a second nucleotide sequence that specifically hybridizes to the complement of nucleotides 1–29,574 of SEQ ID NO. 3 under high stringency conditions and (2) a third nucleotide sequence encoding at least one non-adenoviral polypeptide, wherein said third nucleotide sequence is inserted into the first or second nucleotide sequence in a region that is non-essential to replication of the adenoviral genome in sheep cells.
An adenoviral vector which is capable of replicating autonomously as an adenovirus in sheep cells, comprising (1) a first nucleotide sequenceas set forth in SEQ ID NO. 3 or a second nucleotide sequence that specifically hybridizes to the complement of nucleotides 1–29,574 of SEQ ID NO. 3 under high stringency conditions;
- and (2) a third nucleotide sequence encoding an RNA molecule, wherein said third nucleotide sequence is inserted into the first or second nucleotide sequence in a region that is non-essential to replication of the adenoviral genome in sheep cells.
A plasmid comprising a DNA molecule having the nucleotide sequence as set forth in SEQ ID NO. 3.
A plasmid comprising a DNA molecule having a first nucleotide sequence that specifically hybridizes to nucleotides 1–29574 of SEQ ID NO. 3 under high stringency and a second nucleotide sequence encoding a bacterial origin of replication, wherein the first nucleotide sequence comprises ovine adenovirus inverted terminal repeat sequences that are linked by a third nucleotide sequence which contains at least one unique restriction enzyme site that is not present in the first nucleotide sequence, wherein said third nucleotide sequence is inserted into the first or second nucleotide sequence in a region that is non-essential to replication of the first sequence as an adenovirus in sheep cells, and wherein the first nucleotide sequence is capable of replicating autonomously as an adenovirus in sheep cells.
Dependencies are inferred from the claim text.