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US 7,037,416 B2Granted
Method for monitoring flow rate using fluorescent markers
AssigneeCaliper Life Sciences, Inc.
InventorsJ. Wallace Parce, Claudia B. Jaffe
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Abstract
Microfluidic methods, devices, and systems are provided for monitoring and controlling flow rates in response to one or more marker signals. The marker signals are used to provide an indication of flow rate in the various channels of the devices. Signals obtained from the markers are deconvoluted and used in a feedback loop to make flow rate adjustments.
Claims70
A method of monitoring a flow rate of a fluidic material in a microfluidic device, the method comprising:
- (i) flowing a first marker moiety through the-at least one microscale channel;
- (ii) flowing the fluidic material through the at least one microscale channel;
- (iii) flowing a second marker moiety through the at least one microscale channel;
- (iv) detecting the first marker moiety, resulting in detection of a first signal having, a first area and a first retention time;
- (v) detecting the second marker moiety, resulting in detection of a second signal having a second area and a second retention time;
- and,
- (vi) deconvoluting the first signal and the second signal to provide an indication of the flow rate of the fluidic material, wherein the deconvoluting comprises identifying differences in area and retention time between two or more of:
- the first signal, the second signal, a first selected standard, or a second selected standard.
5 more elements
Dependencies are inferred from the claim text.