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US 7,033,831 B2Granted
Islet cells from human embryonic stem cells
AssigneeGeron Corporation
InventorsGregory J. Fisk, Margaret S. Inokuma
Life of this patent
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Abstract
This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells. High quality populations of islet cells can be produced in commercial quantities for use in research, drug screening, or regenerative medicine.
Claims10
A method for obtaining human insulin secreting cells, comprising
- a) culturing hES cells in a first medium comprising Activin A;
- and
- b) maturing the cells from a) in a second medium comprising nicotinamide;
- thereby obtaining a population comprising human insulin secreting cells.
1 more element
A method for obtaining human insulin secreting cells, comprising
- a) culturing hES cells in a suspension culture to form embryoid bodies;
- b) culturing the cells from a) in a medium comprising trans-retinoic acid;
- c) culturing the cells from b) in a medium comprising a TGF-β antagonist and one or more mitogens;
- and
- d) maturing the cells from c) in a medium comprising nicotinamide;
- thereby obtaining a population comprising human insulin-secreting cells.
3 more elements
A method for obtaining human insulin secreting cells, comprising:
- a) culturing hES cells in a first medium comprising Activin A;
- b) culturing the cells from step a) in a second medium comprising a TGF-β antagonist and one or more mitogens;
- and
- c) culturing the cells from step b) in a third medium comprising nicotinamide;
- thereby obtaining a population comprising human insulin-secreting cells.
2 more elements
Dependencies are inferred from the claim text.