Methods for determining the acetylation level of a peptide based on sensitivity of the peptide to peptidase
AssigneeCyclex, Inc.
InventorsKatsuyuki Tamai, Toshiaki Miyazaki, Emi Wada, Ayumi Tatsuzawa
Life of this patent
Estimating the term…
Abstract
The acetylation level of a peptide is determined utilizing the fact that the changes in the acetylation level are reflected in the sensitivity of the substrate peptide to a peptidase. This method can be used for measuring activities of deacetylase and acetylase, and also enables screening for substances that influence the activity of these enzymes. The deacetylase activity can be measured by a simple procedure according to the present invention.
Claims25
A method for determining an acetylation level of a substrate peptide, comprising:
- (a) contacting a substrate peptide labeled with a substance which emits a signal with a peptidase whose peptide-cleaving activity changes upon the acetylation level of the peptide;
- and
- (b) measuring a quantity of the substrate peptide cleaved by the peptidase based on the signal generated upon cleavage of the substrate peptide by the peptidase, wherein said quantity of the substrate peptide cleaved by the peptidase indicates the acetylation level of the substrate peptide, so that the acetylation level of the substrate peptide is determined.
A method for determining a deacetylation activity of a sample containing a deacetylase, comprising:
- (a) contacting a substrate peptide labeled with a substance which emits a signal with the sample containing the deacetylase under a condition necessary for deacetylation by the deacetylase;
- and
- (b) contacting the substrate peptide of step (a) with a peptidase whose peptide-cleaving activity changes upon an acetylation level of the substrate peptide;
- and
- (c) measuring a quantity of the substrate peptide cleaved by the peptidase of step (b) based on the signal generated upon cleavage of the substrate peptide by the peptidase, wherein said quantity of the substrate peptide cleaved by the peptidase indicates the acetylation level of the substrate peptide and wherein said acetylation level of the substrate peptide indicates the deacetylation activity of the sample containing the deacetylase, so that the deacetylation activity of the sample is determined.
A method for Identifying a compound that inhibits or enhances deacetylation activity of a deacetylase, comprising:
- (a) contacting an acetylated substrate peptide labeled with a substance which emits a signal with the deacetylase in the presence of a test compound under a condition necessary for deacetylation by the deacetylase;
- (b) contacting the substrate peptide of step (a) with a peptidase whose peptide cleaving activity changes upon an acetylation level of the substrate peptide;
- (c) measuring a quantity of the substrate peptide cleaved by the peptidase of step (b) based on the signal generated upon cleavage of the substrate peptide by the peptidase, wherein said quantity of the substrate peptide cleaved by the peptidase indicates the acetylation level of the substrate peptide and wherein said acetylation level of the substrate peptide indicates the deacetylation activity of the deacetylase;
- and
- (d) identifying the compound that decreases or increases the deacetylation activity of the deacetylase relative to the deacetylation activity of the deacetylase in the absence of the test compound.
A method for determining an acetylation activity of a sample containing an acetylase, comprising:
- (a) contacting a substrate peptide labeled with a substance which emits a signal with the sample containing the acetylase under a condition necessary for acetylation by the acetylase;
- (b) contacting the substrate peptide of step (a) with a peptidase whose peptide-cleaving activity changes upon an acetylation level of the substrate peptide;
- (c) measuring a quantity of the substrate peptide cleaved by the peptidase of step (b) based on the signal generated upon cleavage of the substrate peptide by the peptidase, wherein said quantity of the substrate peptide cleaved by the peptidase indicates the acetylation level of the substrate peptide and wherein said acetylation level of the substrate peptide indicates the acetylation activity of the sample containing the acetylase, so that acetylation activity of the sample is determined.
Dependencies are inferred from the claim text.