Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair
AssigneeArtecel Sciences, Inc.
InventorsYuan-Di C. Halvorsen, William O. Wilkison, Jeffrey Martin Gimble
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Abstract
Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either in cultivating monolayers or in a biocompatible lattice or matrix in a three-dimensional configuration. Use of the differentiated chondrocytes for the therapeutic treatment of a number of human conditions and diseases including repair of cartilage in vivo is disclosed.
Claims19
A method of producing a synthetic cartilage patch, the method comprising:
- a) culturing an adipose tissue derived stromal cell in a differentiation medium, wherein said adipose tissue derived stromal cell differentiates into a chondrocytic cell;
- b) introducing said chondrocytic cell to a pre-shaped well, wherein said pre-shaped well is a biocompatible matrix capable of supporting chondro genesis;
- and
- c) culturing said chondrocytic cell in said pre-shaped well, wherein said chondrocytic cell secretes a cartilage-specific extracellular matrix component to produce said synthetic cartilage patch.
Dependencies are inferred from the claim text.