Ketogulonigenium endogeneous plasmids
AssigneeArcher Daniels Midland Company
InventorsJohn D'Elia, Steven F Stoddard
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Abstract
The present invention relates, in general, to a genus of bacteria known as Ketogulonigenium. The present invention further relates to transformed Ketogulonigenium, and methods of transforming Ketogulonigenium. The present invention also relates to nucleic acid molecules, and vectors.
Claims20
An isolated or purified nucleic acid molecule comprising a polynucleotide comprising a replicon functional in Ketogulonigenium, the polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO:
- 3;
- and
- (b) the nucleotide sequence that is the complement to SEQ ID NO:
- 3.
An isolated nucleic acid molecule comprising a polynucleotide comprising a replicon functional in Ketogulonigenium, wherein the polynucleotide hybridizes under stringent hybridization conditions to a sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO:
- 3;
- and
- (b) the nucleotide sequence that is the complement to SEQ ID NO:
- 3;
- wherein said polynucleotide which hybridizes, does not hybridize under stringent hybridization conditions to a polynucleotide having a nucleotide sequence consisting of only A residues or of only T residues.
An isolated or purified nucleic acid molecule comprising a polynucleotide comprising a replicon functional in Ketogulonigenium, the polynucleotide having a nucleotide sequence at least 95% identical to a Ketogulonigenium replicon sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO:
- 7;
- and
- (b) the nucleotide sequence that is the complement to SEQ ID NO:
- 7.
An isolated nucleic acid molecule comprising a polynucleotide comprising a replicon functional in Ketogulonigenium, wherein the polynucleotide hybridizes under stringent hybridization conditions to a sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO:
- 7;
- and
- (b) the nucleotide sequence that is the complement to SEQ ID NO:
- 7;
- wherein said polynucleotide which hybridizes, does not hybridize under stringent hybridization conditions to a polynucleotide having a nucleotide sequence consisting of only A residues or of only T residues.
Dependencies are inferred from the claim text.