US 7,030,215 B2 · Position dependent recognition of GNN nucleotide triplets by zinc fingers

Abstract

The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.

A method for preparing a zinc finger protein comprising a first (F1), a second (F2), and a third (F3) zinc finger, ordered F1, F2, F3 from N-terminus to C-terminus that binds to a target site comprising, in 3′ to 5′ direction, a first (S1), a second (S2), and a third (S3) target subsite, each target subsite having the nucleotide sequence GNN, the method comprising the steps of:

(i) selecting the F1 zinc finger such that it binds to the S1 target subsite, wherein if S1 comprises GAA, F1 comprises the amino acid sequence QRSNLVR (SEQ ID NO:158);
if S1 comprises GAG, F1 comprises the amino acid sequence RSDNLAR (SEQ ID NO:130);
if S1 comprises GAC, F1 comprises the amino acid sequence DRSNLTR (SEQ ID NO:395);
if S1 comprises GAT, F1 comprises the amino acid sequence QSSNLAR (SEQ ID NO:1765);
if S1 comprises GGA, F1 comprises the amino acid sequence QSGHLAR (SEQ ID NO:413);
if S1 comprises GGG, F1 comprises the amino acid sequence RSDHLAR (SEQ ID NO:127);
if S1 comprises GGC, F1 comprises the amino acid sequence DRSHLTR (SEQ ID NO:1506);
if S1 comprises GGT, F1 comprises the amino acid sequence QSSHLTR (SEQ ID NO:835);
if S1 comprises GCA, F1 comprises QSGSLTR (SEQ ID NO:342);
if S1 comprises GCG, F1 comprises RSDDLTR (SEQ ID NO:188);
if S1 comprises GCC, F1 comprises ERGTLAR (SEQ ID NO:131);
if S1 comprises GCT, F1 comprises the amino acid sequence QSSDLTR (SEQ ID NO:1450);
if S1 comprises GTA, F1 comprises the amino acid sequence QSGALTR (SEQ ID NO:1398);
if S1 comprises GTG, F1 comprises the amino acid sequence RSDALTR (SEQ ID NO:153);
if S1 comprises GTC, F1 comprises the amino acid sequence DRSALAR (SEQ ID NO:184);
selecting the F2 zinc finger such that it binds to the S2 target subsite, wherein S2 comprises GAA, F2 comprises the amino acid sequence QSGNLAR (SEQ ID NO:801);
if S2 comprises GAG, F2 comprises the amino acid sequence RSDNLAR (SEQ ID NO:130);
if S2 comprises GAC, F2 comprises the amino acid sequence DRSNLTR (SEQ ID NO:395);
if S2 comprises GAT, F2 comprises the amino acid sequence TSGNLVR (SEQ ID NO:1442);
if S2 comprises GGA, F2 comprises the amino acid sequence QSGHLQR (SEQ ID NO:287);
if S2 comprises GGG, F2 comprises the amino acid sequence RSDHLSR (SEQ ID NO:229);
if S2 comprises GGC, F2 comprises the amino acid sequence DRSHLAR (SEQ ID NO:1092);
if S2 comprises GGT, F2 comprises the amino acid sequence TSGHLSR (SEQ ID NO:1201);
if S2 comprises GCA, F2 comprises the amino acid sequence QSGDLTR (SEQ ID NO:220);
if S2 comprises GCG, F2 comprises the amino acid sequence RSDDLQR (SEQ ID NO:1844);
if S2 comprises GCC, F2 comprises the amino acid sequence DRSDLTR (SEQ ID NO:417);
if S2 comprises GCT, F2 comprises the amino acid sequence QSSDLTR (SEQ ID NO:1450);
if S2 comprises GTA, F2 comprises the amino acid sequence QSGALAR (SEQ ID NO:3339);
if S2 comprises GTG, F2 comprises the amino acid sequence RSDALSR (SEQ ID NO:237);
if S2 comprises GTC, F2 comprises the amino acid sequence DRSALAR (SEQ ID NO:184);
and
selecting the F3 zinc finger such that it binds to the S3 target subsite, wherein if S3 comprises GAA, F3 comprises the amino acid sequence QSGNLAR (SEQ ID NO:801);
if S3 comprises GAG, F3 comprises the amino acid sequence RSDNLTR (SEQ ID NO:231);
if S3 comprises GAC, F3 comprises the amino acid sequence DRSNLTR (SEQ ID NO:395);
if S3 comprises GAT, F3 comprises the amino acid sequence TSANLSR (SEQ ID NO:377);
if S3 comprises GGA, F3 comprises the amino acid sequence QSGHLQR (SEQ ID NO:287);
if S3 comprises GGG, F3 comprises RSDHLSR (SEQ ID NO:229);
if S3 comprises GGT, F3 comprises the amino acid sequence TSGHLVR (SEQ ID NO:1425);
if S3 comprises GCA, F3 comprises the amino acid sequence QSGDLTR (SEQ ID NO:220);
if S3 comprises GCG, F3 comprises the amino acid sequence RSDDLTR (SEQ ID NO:188);
if S3 comprises GCC, F3 comprises the amino acid sequence DRSDLTR (SEQ ID NO:417);
if S3 comprises GCT, F3 comprises the amino acid sequence QSSDLQR (SEQ ID NO:132);
if S3 comprises GTG, F3 comprises RSDALTR (SEQ ID NO:153);
and if S3 comprises GTC, F3 comprises the amino acid sequence DRSALAR (SEQ ID NO:184);
(ii) synthesizing a polynucleotide encoding the protein of (i);
(iii) introducing the polynucleotide of (ii) into a cell;
and
(iv) incubating the cell under conditions in which the protein is expressed;
thereby preparing the zinc finger protein.

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Position dependent recognition of GNN nucleotide triplets by zinc fingers

Life of this patent

Position dependent recognition of GNN nucleotide triplets by zinc fingers

Life of this patent

Abstract

Claims1