Electro-optical device and methods for hybridization and detection
AssigneeAgilent Technologies, Inc.
InventorsGanapati R. Mauze, Dan-Hui Yang
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Abstract
The invention provides an apparatus and method for detection of a target molecule. The apparatus includes a probe labeled with a transition metal-ligand complex that hybridizes with the target to form an initial complex, a metal ion for doping the initial complex and forming a final complex, and a potential means for providing a potential to the final complex to produce a detectable signal indicating the presence of the target after redox reaction. The method of the invention teaches the steps of hybridizing a probe with an attached label to the target to produce an initial complex, adding a metal ion to the initial complex to form a final complex and applying a potential to the final complex to produce a measurable signal.
Claims14
A method for detecting the presence of a target nucleic acid, comprising:
- (a) hybridizing a probe with an attached transition metal ligand complex to said target nucleic acid to produce on initial complex;
- (b) adding a free metal ion to the initial complex to produce an electrically conductive complex;
- and,
- (c) applying an electrical potential to the electrically-conductive complex to produce a measurable light signal.
A method for detecting the presence of a nucleic acid target, comprising:
- independently adding together a probe having an attached transition metal-ligand complex, a target capable of hybridizing to the probe, and a free metal ion to produce an electrically-conductive complex;
- and
- applying an electrical potential to said electrically conductive complex to detect the presence of said nucleic acid target.
A method for detecting the presence of a nucleic acid target, comprising:
- (a) hybridizing a probe having an attached transition metal-ligand complex with said target to produce an initial complex, wherein the transition metal-ligand complex produces a light signal in response to application of an electric potential;
- (b) adding a free metal ion to the initial complex to produce a final electrically conductive complex;
- and
- (c) applying the electric potential through the final complex to the transition metal-ligand complex, to cause the label to produce the light signal.
A method for detecting the presence of a targct nucleic acid, comprising:
- (a) hybridizing a probe attached to a substrate with an attached transition metal ligand complex to said target nucleic acid to produce an initial complex;
- (b) adding a free metal ion to the initial complex to produce an electrically conductive complex;
- and,
- (c) applying an electrical potential to the electrically-conductivc complex to produce a measurable light signal.
A method for detecting the presence of a nucleic acid target, comprising:
- independently adding together a probe attached to a substrate having an attached transition metal-ligand complex, a target capable of hybridizing to the probe, and a free metal on to produce an electrically-conductive complex;
- and
- applying an electrical potential to said electrically conductive complex to detect the presence of said nucleic acid target.
A method for detecting the presence of a nucleic acid target, comprising:
- (a) hybridizing a probe attached to a substrate having an attached transition metal-ligand complex with said target to produce an initial complex, wherein the transition metal-ligand complex produces a light signal in response to application of an electric potential;
- (b) adding a free metal ion to the initial complex to produce a final electrically conductive complex;
- and
- (c) applying the electric potential through the final complex to the transition metal-ligand complex, to cause the label to produce the light signal.
Dependencies are inferred from the claim text.