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AssigneeSyngenta Mogen B.V.
InventorsMaarten Hendrik Stuiver, Anne Silene Ponstein, Stephan Andreas Ohl, Oscar Johanna Maria Goddijn, Lambertus Henricus Simons, Bernardus Martinus Maria Dekker, Sietske Hoekstra, Hendrik Tigelaar, Nicolas Elzinga
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The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.
A vector for plant transformation comprising (i) a T-DNA sequence comprising a sequence located between two direct repeats and (ii) an antisense housekeeping gene, wherein the housekeeping gene is selected from the group consisting of an ATP synthase gene, a cytochrome c gene, a pyruvate kinase gene, an aminoacyl transferase gene, a phosphate translocator gene, a dicarboxylate translocator gene, and a 2-oxo-glutarate translocator gene, wherein said antisense housekeeping gene is not located within said T-DNA sequence.
Dependencies are inferred from the claim text.