Tyrosylprotein sulfotransferases and methods of use thereof
AssigneeSiwa Biotech Corporation
InventorKevin L. Moore
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Abstract
Tyrosylprotein sulfotransferases and nucleic acids encoding the tyrosylprotein sulfotransferases are described. Dual isotopes of the enzyme and of the nucleic acids encoding said enzymes have been identified in human, mouse and C. elegans. The polypeptides and polynucleotides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational tyrosine sulfation of proteins or peptides produced within the expression system. The enzymes can be used to sulfate peptides or proteins requiring sulfation.
Claims31
An isolated polynucleotide which encodes a protein having tyrosylprotein sulfotransferase activity and which is selected from the group consisting of:
- (a) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 82-1194 of SEQ ID NO:2;
- and
- (b) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 289-1086 of SEQ ID NO:2;
- and
- wherein the polynucleotides of (a) and (b) hybridize under conditions comprising prehybridization and hybridization at 68° C. followed by washing twice with two×SSC, 0.1% SDS at 22° C., and washing twice with 0.2×SSC, 0.1% SDS at 22° C.;
- or prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 25% formamide, and washing with 2×SSC, 0.2% SDS at 50° C.
Dependencies are inferred from the claim text.