Methods related to Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
AssigneePfizer Inc.
InventorsKim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
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Abstract
The present invention relates to polynucleotide molecules comprising sequences encoding avec gene products, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of Streptomyces avermitilis. AveC genes, homologs and partial homologs thereof are described for S. avermitilis, S. hygroscopicus, and S. griseochromogenes, respectively. The present invention further relates to vectors, host cells, and mutant strains of Streptomyces avermitilis in which the avec gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
Claims7
An isolated polynucleotide molecule comprising the nucleotide sequence encoding the amino acid sequence of SEQ ID NO:4.
An isolated polynucleotide molecule consisting of the nucleotide sequence of SEQ ID NO:3.
Dependencies are inferred from the claim text.