Streptococcus equisimilis hyaluronan synthase gene and expression thereof in Bacillus subtilis
AssigneeThe Board of Regents of the University of Oklahoma
InventorsPaul H. Weigel, Kshama Kumari, Paul DeAngelis
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Abstract
The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Streptococcus equisimilis hyaluronate synthase (seHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known as hyaluronic acid or hyaluronan.
Claims39
A recombinant host cell, wherein the recombinant host cell is a Bacillus subtilis cell transformed with a recombinant vector comprising a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase, wherein the coding region encodes a single protein that is a dual-action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA.
A recombinant method for producing hyaluronic acid, comprising the steps of:
- introducing a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase into a Bacillus subtilis strain, wherein the coding region encodes a single protein that is a dual-action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA;
- growing the Bacillus subtilis strain in a medium to secrete hyaluronic acid;
- and
- recovering the secreted hyaluronic acid.
Dependencies are inferred from the claim text.