Gabaa receptor epsilon subunits
AssigneeHuman Genome Sciences, Inc.
InventorsMichael C. Hanna, Ewen F. Kirkness
Life of this patent
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Abstract
The present invention relates to a novel GABAA receptor ε subunit (GABRE) and an alternative transcript thereof (ET2). More specifically, isolated nucleic acid molecules are provided encoding human GABRE and ET2 receptor subunits. ET2 and GABRE polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of ET2 and GABRE activities. Also provided are diagnostic methods for detecting aberrant GABRE and ET2 expression, as well as therapeutic methods for treating disorders involving ET2 and GABRE.
Claims39
An isolated polynucleotide encoding a polypeptide comprising an amino acid sequence at least 95% identical to amino acids 1 to 488 of SEQ ID NO:42;
- wherein % identity is determined with parameters that calculate % identity over the full length of amino acids 1 to 488 of SEQ ID NO:42 and that allow gaps of up to 5% of the total number of residues in amino acids 1 to 488 of SEQ ID NO:42;
- wherein said polypeptide forms a GABAA receptor complex with α- and β-GABAA receptor subunits;
- and
- wherein said complex produces GABA-activated chloride currents.
An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising an amino acid sequence at least 95% identical to amino acids −17 to 488 of SEQ ID NO:42;
- wherein % identity is determined with parameters that calculate % identity over the full length of amino acids −17 to 488 of SEQ ID NO:42 and that allow gaps of up to 5% of the total number of residues in amino acids −17 to 488 of SEQ ID NO:42;
- wherein said polypeptide forms a GABAA receptor complex with α- and β-GABAA receptor subunits;
- and
- wherein said complex produces GABA-activated chloride currents.
An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising an amino acid sequence at least 95% identical to amino acids −18 to 488 of SEQ ID NO:42;
- wherein % identity is determined with parameters that calculate % identity over the full length of amino acids −18 to 488 of SEQ ID NO:42 and that allow gaps of up to 5% of the total number of residues in amino acids −18 to 488 of SEQ ID NO:42;
- wherein said polypeptide forms a GABAA receptor complex with α- and β-GABAA receptor subunits;
- and
- wherein said complex produces GABA-activated chloride currents.
An isolated polynucleotide encoding a polypeptide comprising an amino acid sequence at least 95% identical to the complete amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 209642;
- wherein % identity is determined with parameters that calculate % identity over the full length of the complete amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 209642 and that allow gaps of up to 5% of the total number of residues of the complete amino acid sequence encoded by the cDNA clone in ATCC Deposit No. 209642;
- wherein said polypeptide forms a GABAA receptor complex with α- and β-GABAA receptor subunits;
- and
- wherein said complex produces GABA-activated chloride currents.
An isolated polynucleotide comprising 60 contiguous nucleotides of the coding region of SEQ ID NO:41.
Dependencies are inferred from the claim text.