Cancer diagnosis by the measurement of Nup88 in body samples
OwnerHanswalter Zentgraf, Susanne Franz +2inventors
InventorsHanswalter Zentgraf, Susanne Franz, Angel Alonso, Nerea Martinez
Life of this patent
Estimating the term…
Abstract
The invention is directed to a method of diagnosis of the existence and/or the pathological developmental stage and/or the grade of malignancy of carcinomas and/or sarcomas in mammals characterized by the preparation of a body sample of said mammals and the determination of the overexpression of the protein Nup88 in said sample and to antibodies suitable for performing the same.
Claims9
A monoclonal antibody as deposited under DSM ACC 2457.
A method for identifying the presence of a malignant cancer cell comprising:
- (a) providing a tissue biopsy sample;
- and (b) determining the level of expression in the tissue biopsy sample of the protein consisting of the amino acid sequence (SEQ ID NO:
- 2):
- MAAAEGPVGDGELWQTWLPNHVVFLRLREGLKNQSPTEAEKPASSSL
- PSSPPPQLLTRNVVFGLGGELFLWDGEDSSFLVVRLRGPSGGGEEPAL
- SQYQRLLCINPPLFEIYQVLLSPTQHHVALIGIKGLMVLELPKRWGKNSE
- FEGGKSTVNCSTTPVAERFFTSSTSLTLKHAAWYPSEILDPHVVLLTSD
- NVIRIYSLREPQTPTNVIILSEAEEESLVLNKGRAYTASLGETAVAFDFG
- PLDAVPKTLFGQNGKDEVVAYPLYILYENGETFLTYISLLHSPGNIWKAV
- GSIAHASAAEDNYGYDACAVLCLPCVPNILVIATESGMLYHCVVLEGEEE
- DDHTSEKSWDSRIDLIPSLYVFECVELELALKLASGEDDPFDSDFSCPVK
- LHRDPKCPSRYHCTHEAGVHSVGLTWIHKLHKFLGSDEEDKDSLQELST
- EQKCFVEHILCTRPLPCRQPAPIRGFWIVPDILGPTMICITSTYECLIWP
- LLSTVHPASPPLLCTREDVEVAESSLRVLAETPDSFEKHIRSILQRSVAN
- PAFLKASEKDIAPPPEECLQLLSRATQVFREQYILKQDLAKEEIQRRVKL
- LCDQKKKQLEDLSYCREERKSLREMAERLADKYEEAKEKQEDIMNRMKKL
- LHSFHSELPVLSDSERDMKKELQLIPDQLRHLGNAIKQVTMKKDYQQQK
- MEKVLSLPKPTIILSAYQRKCIQSILKEEGEHIREMVKQINDIRNHVNF,
- by contacting the sample with the monoclonal antibody deposited under DSM ACC 2457, which binds to the protein, wherein the tissue biopsy sample comprises a malignant cancer cell if the expression level of the protein is greater than the expression level of that protein in healthy or normal control tissue.
A kit for diagnosing the presence of a malignant cancer cell, comprising the monoclonal antibody deposited under DSM ACC 2457, which binds to the protein consisting of the amino acid sequence (SEQ ID NO:
- 2):
- MAAAEGPVGDGELWQTWLPNHVVFLRLREGLKNQSPTEAEKPASSSLPSSPPPQLLT
- RNVVFGLGGELFLWDGEDSSFLVVRLRGPSGGGEEPALSQYQRLLCINPPLFEIYQVLLSPTQ
- HHVALIGIKGLMVLELPKRWGKNSEFEGGKSTVNCSTTPVAERFFTSSTSLTLKHAAWYPSEI
- LDPHVVLLTSDNVIRIYSLREPQTPTNVIILSEAEEESLVLNKGRAYTASLGETAVAFDFGPLD
- AVPKTLFGQNGKDEVVAYPLYILYENGETFLTYISLLHSPGNIWKAVGSIAHASAAEDNYGY
- DACAVLCLPCVPNILVIATESGMLYHCVVLEGEEEDDHTSEKSWDSRIDLIPSLYVFECVELE
- LALKLASGEDDPFDSDFSCPVKLHRDPKCPSRYHCTHEAGVHSVGLTWIHKLHKFLGSDEED
- KDSLQELSTEQKCFVEHILCTRPLPCRQPAPIRGFWIVPDILGPTMICITSTYECLIWPLLSTVHP
- ASPPLLCTREDVEVAESSLRVLAETPDSFEKHIRSILQRSVANPAFLKASEKDIAPPPEECLQLL
- SRATQVFREQYILKQDLAKEEIQRRVKLLCDQKKKQLEDLSYCREERKSLREMAERLADKY
- EEAKEKQEDIMNRMKKLLHSFHSELPVLSDSERDMKKELQLIPDQLRHLGNAIKQVTMKKD
- YQQQKMEKVLSLPKPTIILSAYQRKCIQSILKEEGEHIREMVKQINDIRNHVNF.
A method for identifying the presence of a malignant cancer cell comprising (a) providing a tissue biopsy sample;
- and (b) determining the level of expression in the tissue biopsy sample of a protein consisting of the amino acid sequence depicted in SEQ ID NO:
- 2 by contacting the sample with at least one of (i) a monoclonal antibody bearing the accession number DSM ACC 2457 and (ii) a recombinant or chimeric molecule that comprises at least six CDRs of the monoclonal antibody bearing the accession number DSM ACC 2457, which binds to the protein, wherein the tissue biopsy sample comprises a malignant cancer cell if the expression level of the protein is determined to be greater than the expression level of that protein in healthy or normal control tissue.
Dependencies are inferred from the claim text.