Amyloid-β protein aggregation-regulating factors
AssigneeFujisawa Pharmaceutical Co., Ltd.
InventorsToshio Ota, Takao Isogai, Tetsuo Nishikawa, Yuri Hio, Mayako Yamazaki, Susumu Satoh, Hiroyuki Arakawa, Masahiko Morita
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Abstract
The present invention provides: proteins suppressing or promoting the aggregation or deposition of amyloid-β protein; polynucleotides encoding the proteins; a method for screening a compound suppressing or promoting the aggregation or deposition of amyloid-β protein; and therapeutic agents for treating or preventing Alzheimer's diseases comprising a compound that regulates the activity of a protein suppressing or promoting the aggregation or deposition of amyloid-β protein.
Claims18
A polypeptide which (a) suppresses or promotes the aggregation of
- amyloid-β or (b) which is an immunogenic polypeptide, and which is encoded by an isolated polynucleotide selected from the group consisting of:
- (a) a polynucleotide that comprises a polynucleotide sequence as set forth in SEQ ID NO:
- 3, 5, 7 or 9,
- (b) a polynucleotide that encodes a polypeptide having an amino acid sequence as set forth in SEQ ID NO:
- 4, 6, 8 or 10,
- (c) a polynucleotide that hybridizes under stringent conditions to a polynucleotide comprising a polynucleotide sequence as set forth in SEQ ID NO:
- 3, 5, 7 or 9, wherein the stringent conditions comprise washing in 0.1×SSC and 0.1% SDS at 65° C.,
- (d) a polynucleotide that shows at least 95% homology to a polynucleotide sequence as set forth in SEQ ID NO:
- 3, 5, 7 or 9, and
- (e) a fragment of the polynucleotide of (a), (b), (c) or (d).
An immunological assay comprising:
- contacting the polypeptide of claim 1 with a sample containing an antibody, and
- monitoring the binding of antibody in said sample with the polypeptide of claim 1,
- wherein binding of antibodies in said sample to said polypeptide is indicative of the presence of antibodies that immunologically react with the polypeptide of claim 18 or inhibit its ability to suppress or promote the aggregation or deposition of amyloid β protein.
Dependencies are inferred from the claim text.