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AssigneeH. Lundbeck A/S
InventorsJames A. Bonini, Gabriel S. Lerman, Kristine L. Ogozalek, Yong Quan
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This invention provides isolated nucleic acids encoding mammalian SNORF62 and SNORF72 receptors, purified mammalian SNORF62 and SNORF72 receptors, vectors comprising nucleic acid encoding mammalian SNORF62 and SNORF72 receptors, cells comprising such vectors, antibodies directed to mammalian SNORF62 and SNORF72 receptors, nucleic acid probes useful for detecting nucleic acid encoding mammalian SNORF62 and SNORF72 receptors, antisense oligonucleotides complementary to unique sequences of nucleic acid encoding mammalian SNORF62 and SNORF72 receptors, transgenic, nonhuman animals which express DNA encoding normal or mutant mammalian SNORF62 and SNORF72 receptors, methods of isolating mammalian SNORF62 and SNORF72 receptors, methods of treating an abnormality that is linked to the activity of the mammalian SNORF62 and SNORF72 receptors, as well as methods of determining binding of compounds to mammalian SNORF62 and SNORF72 receptors, methods of identifying agonists and antagonists of SNORF62 and SNORF72 receptors, and agonists and antagonists so identified. This invention also provides methods of treating an abnormality that is linked to the activity of a mammalian NMU receptor, as well as methods of determining binding of compounds to mammalian NMU receptors, methods of identifying agonists and antagonists of NMU receptors, and agonists and antagonists so identified.
A process involving competitive binding for identifying a chemical compound which specifically binds to a mammalian SNORF72 receptor which comprises separately contacting cells, or a membrane preparation from such cells, expressing on their cell surface the mammalian SNORF72 receptor, wherein such cells do not normally express the mammalian SNORF72 receptor, with both the chemical compound and a second chemical compound known to bind to the receptor, and with only the second chemical compound, under conditions suitable for binding of such compounds to the receptor, and detecting specific binding of the chemical compound to the mammalian SNORF72 receptor, a decrease in the binding of the second chemical compound to the mammalian SNORF72 receptor in the presence of the chemical compound being tested indicating that such chemical compound binds to the mammalian SNORF72 receptor;
A method of screening a plurality of chemical compounds not known to bind to a mammalian SNORF72 receptor to identify a compound which specifically binds to the mammalian SNORF72 receptor, which comprises
A process for determining whether a chemical compound is a mammalian SNORF72 receptor antagonist which comprises contacting cells transfected with and expressing DNA encoding the mammalian SNORF72 receptor with the compound in the presence of a known mammalian SNORF72 receptor agonist, under conditions permitting the activation of the mammalian SNORF72 receptor, and detecting any decrease in mammalian SNORF72 receptor activity, so as to thereby determine whether the compound is a mammalian SNORF72 receptor antagonist;
A process for determining whether a chemical compound specifically binds to and inhibits activation of a mammalian SNORF72 receptor, which comprises separately contacting cells producing a second messenger response and expressing on their cell surface the mammalian SNORF72 receptor, wherein such cells do not normally express the mammalian SNORF72 receptor, with both the chemical compound and a second chemical compound known to activate the mammalian SNORF72 receptor, and with only the second chemical compound, under conditions suitable for activation of the mammalian SNORF72 receptor, and measuring the second messenger response in the presence of only the second chemical compound and in the presence of both the second chemical compound and the chemical compound, a smaller change in the second messenger response in the presence of both the chemical compound and the second chemical compound than in the presence of only the second chemical compound indicating that the chemical compound inhibits activation of the mammalian SNORF72 receptor;
A method of screening a plurality of chemical compounds not known to inhibit the activation of a mammalian SNORF72 receptor to identify a compound which inhibits the activation of the mammalian SNORF72 receptor, which comprises:
Dependencies are inferred from the claim text.