DNAs and proteins or peptides specific to bacteria of the species Neisseria meningitidis, processes for obtaining them and their biological uses
AssigneesInstitut National De La Sante Et De La Recherche Medicale (Inserm), Max-Planck-Gesellschaft zur Forderung des Wessenschaften E.V., SmithKline Beecham Corporation
InventorsXavier Nassif, Colin Tinsley
Life of this patent
Estimating the term…
Abstract
The DNA of the invention are characterised in that they concern the whole or part of genes, with their reading frame, to be found in Neisseria meningitidis, but not in Neisseria gonorrhoeae, or in Neisseria lactamica except the genes involved in the biosynthesis of the polysaccharide capsule, frp A, frp C, opc, por A, rotamase the sequence IC1106, IgA protease, pilline, pilC, transferrin binding proteins and opacity proteins. The invention also concerns the polypeptides corresponding to these DNA and the antibodies directed against these polypeptides. It is applicable in the prevention and the detection of meningococcus induced infections and meningitis.
Claims8
An isolated DNA which is specific to Neisseria meningitidis (Nm) and Neisseria gonorrheae (Ng), and hybridizes on a Southern blot to SEQ ID NO:95 and does not hybridize on a Southern blot to a DNA sequence of Neisseria lactamica (Nl) strain Nl8064, under the following hybridization conditions:
- 18 h at 65° C., with a solution comprising 0.5 M NaPO4 pH 7.2, 0.001 M EDTA-Na, 1% bovine serum albumin and 7% sodium dodocylsulphate, followed by at least two washes in a solution comprising 40 mM Na PO4 pH 7.2, 1 mM EDTA, and 1% SDST, the final wash being conducted at 85° C. for 5 minutes, or the complement of said isolated DNA which is specific to Neisseria meningitidis (Nm) and Neisseria gonorrheae (Ng),
- provided that said DNA or the complement of said isolated DNA is not pilC, or a gene involved in the biosynthesis of any one of the polysaccharide capsule, IgA proteases, pilin, a protein which binds transferin, a protein which binds lactoferin, and an opacity protein
- said DNA being within an islet involved in the polonization of the nasopharynx or invasion of the submucousal space or systemic dissemination of Nm.
An isolated DNA which is specific to Neisseria memingitidis (Nm) and Neisseria gonorrheae (Ng), and hybridizes on a Southern blot to SEQ ID NO:95 and does not hybridize on a Southern blot to a DNA sequence of Neisseria lactamica (Nl) strain Nl8064, under the following hybridization conditions:
- 16 h at 65° C., with a solution comprising 0.5 M NaPO4, pH 7.2, M EDTA-Na, 1% bovine serum albumin and 7% sodium dodecylsulphate, followed by at least two washes in a solution comprising 40 mM Na PO4 pH7.2, 1 mM EDTA and 1% SDS, the final wash being conducted at 65° C. for 5 minutes, or the complement of said isolated DNA which is specific to Neisseria meningitidis (Nm) and Neisseria gonorrheae (Ng),
- provided that said DNA or the complement of said isolated DNA is not pilC, or a gene involved in the biosynthesis of any one of the polysaccharide capsule, IgA proteases, pilin, a protein which binds transferrin, a protein which binds lactoferin, and an opacity protein.
Dependencies are inferred from the claim text.