Methods of isolation of amyloid inhibitory ingredients within Uncaria tomentosa
AssigneeUniversity of Washington
InventorsGerardo Castillo, Alan D. Snow
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Abstract
A method for isolating amyloid inhibitory constituents within Uncaria tomentosa plant matter. The method includes the steps of extracting the plant matter with a non-aqueous organic solvent, concentrating the extract, removing insoluble materials, precipitating amyloid inhibitory constituents with organic solvent, recovering and redissolving the amyloid inhibitory constituents obtained in organic solvent, and injecting and separation by HPLC.
Claims28
A method for isolating amyloid inhibitory constituents within Uncaria tomentosa plant matter, the method comprising the following steps:
- a) extracting the plant matter with an non-aqueous organic solvent, b) concentrating the extract, c) removing insoluble materials, d) precipitating amyloid inhibitory constituents with organic solvent e) recovering and redissolving the amyloid inhibitory constituents obtained in organic solvent, and f) injecting and separation by HPLC.
A method for isolating amyloid inhibitory constituents within Uncaria tomentosa plant matter, the method comprising the following steps:
- a) extracting the plant matter with an organic solvent, b) concentrating the extract, c) removing insoluble materials, d) precipitating amyloid inhibitory constituents with organic solvent e) recovering and redissolving the amyloid inhibitory constituents obtained in organic solvent, and f) injecting and separation by HPLC;
- wherein the amyloid inhibitory ingredients within Uncaria tomentosa are identified to be contained within an approximate HPLC retention time of 26 minutes.
Dependencies are inferred from the claim text.