Multiple and multivalent DNA vaccines in ovo

A method for vaccinating a fowl egg comprising:

• injecting into an amniotic fluid of said fowl egg a multivalent DNA vaccine, said multivalent DNA vaccine comprising:
• a DNA construct containing two or more DNA molecules ligated to a vector, wherein each of said DNA molecules contains a gene or a fragment thereof encoding an antigenic peptide of an avian virus causing an avian viral disease in fowl;
• wherein said antigenic peptide of said avian virus is capable of inducing a protective immune response against said avian viral disease in said fowl;
• wherein said vector is a plasmid or a viral carrier;
• wherein said viral carrier is selected from the group consisting of a bacteriophage, an SV40, an adenovirus, a polyoma virus, a vaccinia virus, and a baculovirus;
• and
• wherein said avian virus is selected from the group consisting of Marek's disease virus (MDV), infectious bursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian leucosis virus (ALV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).

A method for delivering a multiple DNA vaccine to a fowl, the method comprising:

• injecting said multiple DNA vaccine into an amniotic fluid of an egg of said fowl, the multiple DNA vaccine comprising:
• two or more DNA constructs, each of said DNA constructs containing a DNA molecule and a vector;
• wherein said DNA molecule comprises a gene or a fragment thereof encoding an antigenic peptide of an avian virus causing an avian viral disease in fowl;
• wherein said antigenic peptide of said avian virus is capable of inducing a protective immune response against said avian viral disease in said fowl;
• wherein said vector is a plasmid or a viral carrier;
• wherein said viral carrier is selected from the group consisting of a bacteriophage, an SAV40, an adenovirus, a polyoma virus, a vaccinia virus, a baculovirus, and a pox virus;
• and
• wherein said avian virus is selected from the group consisting of Marek's disease virus (MDV), infectious bursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian leucosis virus (ALV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).

A method for delivering a multivalent DNA vaccine to a fowl, the method comprising:

• injecting said multivalent DNA vaccine into an amniotic fluid of an egg of said fowl, said multivalent DNA vaccine comprising:
• a DNA construct containing two or more DNA molecules ligated to a vector, wherein each of said DNA molecules contains a gene or a fragment thereof encoding an antigenic peptide of an avian virus causing an avian viral disease in fowl;
• wherein said antigenic peptide of said avian virus is capable of inducing a protective immune response against said avian viral disease in said fowl;
• wherein said vector is a plasmid or a viral carrier;
• wherein said viral carrier is one selected from the group consisting of a bacteriophage, an SV40, an adenovirus, a polyoma virus, a vaccinia virus, and a baculovirus;
• and
• wherein said avian virus is one selected from the group consisting of Marek's disease virus (MDV), infectious bursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian leucosis virus (ALV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).

A method for vaccinating a fowl egg comprising:

• injecting into an amniotic fluid of said fowl egg a multivalent DNA vaccine, said multivalent DNA vaccine comprising:
• a DNA construct containing two or more DNA molecules ligated to a vector, wherein each of said DNA molecules contains a gene or a fragment thereof encoding an antigenic peptide of an avian virus causing an avian viral disease in fowl;
• wherein said antigenic peptide of said avian virus is capable of inducing a protective immune response against said avian viral disease in said fowl;
• wherein said vector is a plasmid;
• and
• wherein said avian virus is one selected from the group consisting of Marek's disease virus (MDV), infectious bursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian leucosis virus (ALV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).

A method for delivering a multivalent DNA vaccine to a fowl, the method comprising:

• injecting said multivalent DNA vaccine into an amniotic fluid of an egg of said fowl, said multivalent DNA vaccine comprising:
• a DNA construct containing two or more DNA molecules ligated to a vector, wherein each of said DNA molecules contains a gene or a fragment thereof encoding an antigenic peptide of an avian virus causing an avian viral disease in fowl;
• wherein said antigenic peptide of said avian virus is capable of inducing a protective immune response against said avian viral disease in said fowl;
• wherein said vector is a plasmid;
• and
• wherein said avian virus is one selected from the group consisting of Marek's disease virus (MDV), infectious bursal disease virus (IBDV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), avian leucosis virus (ALV), duck hepatitis virus (DHV), and hemorrhagic enteritis virus (HEV).