DNA encoding Erwinia amylovora hypersensitive response elicitor and its use
AssigneeCornell Research Foundation, Inc.
InventorsJihyun Francis Kim, Steven V. Beer
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Abstract
The present invention is directed to an isolated DNA molecule from Erwinia amylovora that encodes a protein or polypeptide which elicits a hyersensitive response in plants. This isolated DNA molecule can used to impart disease resistance to plants, to enhance plant growth, and/or to control insects on plants. Plants or plant seeds transformed with a DNA molecule encoding a hypersensitive response elicitor protein or polypeptide can be provided and the transgenic plants or plants resulting from the transgenic plant seeds are grown under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds.
Claims18
An isolated DNA molecule encoding a hypersensitive response eliciting protein or polypeptide, wherein the isolated DNA molecule is selected from the group consisting of (a) a DNA molecule comprising SEQ ID NO:
- 1 and (b) a DNA molecule encoding a protein comprising SEQ ID NO:
- 2, or an isolated DNA molecule complementary to DNA molecules (a) or (b).
An isolated DNA molecule of an Erwinia pathogen, wherein the isolated DNA molecule both encodes a polypeptide that elicits a hypersensitive response in non-host plants, and hybridizes to a DNA molecule comprising the complement of SEQ ID NO:
- 1 under hybridization conditions comprising hybridization at 50° C. for 24 hours in a solution that comprises 6×SSC and 0.5% SDS, followed by wash conditions comprising a first wash at 45° C. in a solution that comprises 2×SSC and a second wash at 45° C. in a solution that comprises 0.1×SSC.
Dependencies are inferred from the claim text.