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US 7,029,665 B2Granted
Human suppressor tRNA oligonucleotides and methods for use for same
AssigneeHuman Gene Therapy Research Institute
InventorsRekha G. Panchal, Charles J. Link, Jr.
Life of this patent
Estimating the term…
Abstract
Novel synthetic suppressor tRNA have been provided which provide read-through of internal nonsense mutations, or which can site-specifically alter translation of transcribed sequences. Uses of the same are also provided in genetic engineering protocols including gene therapy treatment of diseases such as Xeroderma pigmentosum.
Claims14
An isolated oligonucleotide which encodes a synthetic suppressor tRNA comprising:
- (A) a human tRNA structural gene sequence comprising no more that twenty 3′ flanking residues and no 5′ flanking residues, said sequence encoding an anticodon region for pairing with mRNA;
- (B) an anticodon sequence contained within said anticodon region which has been modified to recognize a nonsense mutation;
- wherein said oligonucleotide has a total length of less than 150 nucleotides.
An isolated oligonucleotide which encodes a synthetic suppressor tRNA comprising:
- A) a human tRNA structural gene sequence comprising no more than twenty 3′ flanking residues and no 5′ flanking residues, said sequence encoding an anticodon region for pairing with mRNA;
- B) an anticodon sequence contained within said anticodon region which has been modified to recognize a codon different from that which is originally recognized;
- wherein said oligonucleotide has a sequence selected from the group consisting of SEQ ID NOS:1–10 and wherein said oligonucleotide has a total length of less than 150 nucleotides.
Dependencies are inferred from the claim text.