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US 7,029,629 B2Granted
Computerized method and apparatus for analyzing amino acids
AssigneeHitachi, Ltd.
InventorsYoshio Fujii, Masahito Ito, Kimiyoshi Koda
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Abstract
A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before β-aminoisobutyric acid (β-AiBA) is eluted.
Claims11
An apparatus for analyzing a plurality of amino acids in a fluid sample by a user comprising:
- a container for supplying a buffer solution;
- a control valve for controlling a lithium ion concentration and pH of said buffer solution;
- an auto sampler for supplying said fluid sample;
- a separation column for separating said plurality amino acids in said buffer fluid sample;
- a processor in communication with said control valve and said auto sampler;
- and
- an analysis program residing on said processor for causing said processor to perform the acts of:
- introducing said sample into a buffer solution;
- passing said sample in said buffer solution through a separation column;
- setting a lithium ion concentration in said buffer to no more than 0.3 mols/L up to a time before β-aminoisobutyric acid (β-AiBA) is eluted;
- and
- displaying said analysis for said user.
9 more elements
An apparatus for analyzing a plurality of amino acids in a fluid sample by a user comprising:
- a container for supplying a buffer solution;
- a control valve for controlling a lithium ion concentration and pH of said buffer solution;
- an auto sampler for supplying said fluid sample;
- a separation column for separating said plurality amino acids in said buffer fluid sample;
- a processor in communication with said control valve and said auto sampler;
- and
- an analysis program residing on said processor for causing said processor to perform the acts of:
- introducing said sample into a buffer solution;
- passing said sample in said buffer solution through a separation column;
- setting a lithium ion concentration in said buffer to no more than 0.3 mols/L up to a time before β-aminoisobutyric acid (β-AiBA) is eluted;
- setting a pH to no more than 3.5 for said buffer solution up to a time before said β-aminoisobutyric acid (β-AiBA) is eluted;
- and
- displaying said analysis for said user.
10 more elements
Dependencies are inferred from the claim text.