Sealing interface for microfluidic device

A fluidic system comprising:

• a microfluidic device defining a fluid flow path, the device comprising a plurality of device layers including a first cover layer, a second cover layer, and a plurality of channel-defining stencil layers disposed between the first cover layer and the second cover layer, the first cover layer defining a plurality of fluid ports and the plurality of stencil layers including a first stencil layer disposed adjacent to the first cover layer;
• a fluidic seal adapted to seal against the first cover layer;
• a compression element adapted to move any of the fluidic seal and the first cover layer;
• wherein the first cover layer has a first thickness, the first stencil layer defines a first microfluidic channel having a first width and in fluid communication with a first port of the plurality of fluid ports, the first width being less than about two times the first thickness;
• and
• wherein the fluidic seal engages the first cover layer and exerts a contact pressure of at least about 10 pounds per square inch against at least a portion of the first cover layer without substantially occluding the fluid flow path.

A fluidic processing method comprising the steps of:

• providing a microfluidic device having an outer surface defining a fluidic port, the device defining a fluid flow path and defining a first microfluidic channel having a first width and being disposed within the device at a first depth relative to the outer surface, wherein the first width is less than about two times the first depth;
• providing a fluidic seal adapted to seal against the outer surface adjacent to the fluidic port;
• and
• establishing a contact pressure between at least a portion of the fluidic seal and at least a portion of the outer surface of at least about 10 pounds per square inch without substantially occluding the fluid flow path.