# US 7,026,121 B1 · Methods and compositions for diagnosing and monitoring transplant rejection

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- Number: US 7,026,121 B1
- Document ID: US7026121B1
- Status: Granted
- Kind: B1
- Application number: 10/131,831
- Filed: 2002-04-24
- Granted: 2006-04-11
- Earliest claimed: 2001-06-08
- Assignees: Expression Diagnostics, Inc.
- Inventors: Jay Wohlgemuth; Kirk Fry; Robert Woodward; Ngoc Ly
- Web page: https://patentgrep.com/patent/US7026121B1

## CPC

- C12Q 1/6883: for diseases caused by alterations of genetic material
- C12Q 2600/158: Expression markers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Methods of diagnosing or monitoring transplant rejection or cytomegalovirus infection in a patient by detecting the expression level of one or more genes or surrogates derived therefrom in the patient are described. Diagnostic oligonucleotides for diagnosing or monitoring transplant rejection or cytomegalovirus infection and kits or systems containing the same are also described.

## Claims

### Claim 1

A method of diagnosing or monitoring transplant rejection in a patient, comprising detecting the expression level of a gene in said patient to diagnose or monitor transplant rejection in said patient wherein said gene comprises the nucleotide sequence of SEQ ID NO: 873.

### Claim 2 (depends on 1)

The method of claim 1 wherein said transplant rejection is cardiac transplant rejection.

### Claim 3 (depends on 1)

The method of claim 1 wherein said expression level is detected by measuring the RNA level expressed by said gene.

### Claim 4 (depends on 3)

The method of claim 3 further comprising isolating RNA from said patient prior to detecting the RNA level expressed by said gene.

### Claim 5 (depends on 3)

The method of claim 3 wherein said RNA level is detected by PCR.

### Claim 6 (depends on 3)

The method of claim 3 wherein said RNA level is detected by hybridization.

### Claim 7 (depends on 3)

The method of claim 3 wherein said RNA level is detected by hybridization to an oligonucleotide.

### Claim 8 (depends on 7)

The method of claim 7 wherein the nucleotide sequence comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.