Transiently dynamic flow cytometer analysis system

A flow cytometer comprising:

• a. a fluid stream;
• b. at least one particle entrained in said fluid stream;
• c. an oscillator responsive to said fluid stream;
• d. a first sensor responsive to said at least one particle entrained in said fluid stream;
• e. at least one signal generator;
• f. data from said at least one signal generator incident to a first occurrence;
• g. data from said at least one signal generator incident to at least one additional occurrence;
• h. a signal processor of said data from said at least one signal generator;
• i. a transformation operation applied to at least a portion of said data from said at least one signal generator incident to said first occurrence;
• j. a transformation operation applied to at least a portion of said data from said at least one signal generator incident to said at least one additional occurrence;
• k. a compensated parameter shared by said first occurrence and by said at least one additional occurrence;
• l. a particle differentiation element configured to differentiate said first occurrence from said at least one additional occurrence;
• n. a particle assignment element responsive to said particle differentiation element;
• o. a particle separator based upon an assignment from said particle assignment element;
• and
• p. at least one container in which separated particles are collected.

A method of flow cytometry, comprising the steps of:

• a. establishing a fluid stream;
• b. perturbing said fluid stream;
• c. sensing an occurrence incident to said fluid stream;
• d. generating a signal from said occurrence;
• e. processing said signal using a first signal processor;
• f. processing said signal using at least one additional signal processor in parallel with said first signal processor;
• g. utilizing at least a portion of code by said first signal processor and said at least one additional signal processor;
• h. combining output from said first signal processor and said at least one additional signal processor;
• and
• i. applying said combined output to classify said occurrence.

A flow cytometer comprising:

• a. a fluid stream;
• b. a sensor responsive to an occurrence incident to said fluid stream;
• c. at least one signal generator coupled to said sensor;
• d. a first signal processor to perform operations on signal data from said at least one signal generator;
• e. a second signal processor to perform operations on said signal data from said at least one signal generator;
• f. compensated parameter output from said second signal processor returned to said first signal processor;
• and
• g. a particle differentiation element responsive to said compensated parameter output.

A method of flow cytometry, comprising the steps of:

• a. establishing a fluid stream;
• b. generating a first signal incident to said fluid stream;
• c. generating at least one additional signal incident to said fluid stream;
• d. applying parameter compensation transformation to said first signal and to said at least one additional signal;
• e generating a first compensated signal and at least one additional compensated signal;
• f. comparing said first compensated signal and said at least one additional compensated signal;
• and
• g. classifying occurrences based upon comparison of said first compensated signal and said at least one additional compensated signal.

A flow cytometer comprising:

• a. a fluid stream;
• b. a sensor responsive to an occurrence incident to said fluid stream;
• c. at least one signal generator coupled to said sensor;
• d. a first signal generated by said at least one signal generator;
• e. at least one additional signal generated by said at least one signal generator;
• f. a signal processor responsive to said first signal and to said at least one additional signal;
• g. a transformation operation applied to said first signal and to said at least one additional signal to create compensated parameters;
• h. a compensated parameter comparison element configured to compare said compensated parameters;
• and
• i. a particle differentiation element responsive to said compensated parameter comparison element.

A method of flow cytometry, comprising the steps of:

• a. establishing a fluid stream;
• b. perturbing said fluid stream;
• c. sensing serial occurrences incident to said fluid stream;
• d. generating an analog signal corresponding to said serial occurrences;
• e. performing compensation transformation on said analog signal corresponding to said serial occurrences;
• and
• f. minimizing variations of said analog signal corresponding to said serial occurrences, wherein variations are selected from the group consisting of phase and shape.

A method of flow cytometry, comprising the steps of:

• a. establishing a fluid stream;
• b. entraining a substance in said fluid stream;
• c. perturbing said fluid stream;
• d. sensing serial occurrences incident to said substance, wherein said serial occurrences have a rate of 50,000 per second or greater;
• e. generating a signal from said serial occurrences;
• f. digitizing said signal;
• g. interpreting said serial occurrences using said digitized signal;
• h. saving said digitized signal in a memory storage element;
• and
• i. duplicating said digitized signal saved in said memory storage element.

A method of flow cytometry, comprising the steps of:

• a. establishing a fluid stream;
• b. sensing an occurrence incident to said fluid stream;
• c. generating an original signal from said occurrence;
• d. saving said original signal in a memory storage element;
• e. duplicating said original signal;
• f. processing said duplicate signal of said original signal;
• g. interpreting said occurrence using said processed duplicate signal.

A flow cytometer, comprising:

• a. a fluid stream;
• b. an occurrence incident to said fluid stream;
• c. a sensor responsive to said occurrence incident to said fluid stream;
• d. at least one signal generator coupled to said sensor;
• e. signal data generated by said at least one signal generator;
• f. a memory element to store said signal data;
• g. a stored signal data retrieval element configured to retrieve said stored signal data from said memory element;
• h. a stored signal duplicator configured to duplicate said stored signal data;
• and
• i. at least one signal processor responsive to a duplicated signal from said stored signal duplicator.