Knockin gene-mutated mouse comprising a mutant presenilin-1 gene
AssigneeDaiichi Pharmaceutical Co., Ltd.
InventorsMasatoshi Takeda, Junji Takeda
Life of this patent
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Abstract
A gene-mutated animal such as a mouse which comprises a mutant prsenilin-1 gene comprising a DNA having a sequence encoding a mutant presenilin-1 protein in which an amino acid is substituted with a different amino acid in an amino acid sequence of a presenilin-1 protein; for example, a mutant presenilin protein in which isoleucine at position 213 is substituted with an amino acid other than isoleucine, e.g., threonine, in a mouse presenilin-1 protein. The animal is useful as an animal model which has pathological conditions closer to a human patient with Alzheimer's disease.
Claims17
A knockin gene-mutated mouse having a mutant presenilin-1 gene, wherein the mutant presenilin-1 gene results in overexpression of Amyloid β 42 in the brain of said mouse.
A knockin gene-mutated mouse having a mutant presenilin-1 gene which comprises a DNA having a sequence encoding a mutant mouse presenilin-1 protein in which isoleucine at position 213 of a mouse presenilin-1 protein as set forth in SEQ ID NO:
- 3 is substituted with an amino acid other than isoleucine, wherein the mutant presenilin-1 gene results in overexpression of Amyloid β 42 in the brain of said mouse.
A knockin gene-mutated mouse having a mutant presenilin-1 gene which comprises a DNA having a sequence encoding a mutant mouse presenilin-1 protein in which isoleucine at position 213 of a mouse presenilin-1 protein as set forth in SEQ ID NO:
- 3 is substituted with threonine, wherein the mutant presenilin-1 gene results in overexpression of Amyloid β 42 in the brain of said mouse.
A gene-mutated mouse having a mutant presenilin-1 gene which encodes for the OS-2 type mutation of presenilin-1, wherein the mutant presenilin-1 gene results in overexpression of Amyloid β42 in the brain of said mouse.
An embryo introduced with a plasmid comprising a DNA represented by the nucleotide sequence:
- 5′-TGTGGTCGGGATGATYGCCACCCACTGGAAAGGCCC-3′ (SEQ ID NO:
- 19)
- wherein Y represents T or C.
A method for producing a gene-mutated mouse, wherein the method comprises the step of transferring a mutant presenilin-1 gene by homologous recombination into an embryo of a mouse, wherein the mutant presenilin-1 gene is capable of expressing a mutant presenilin-1 protein and inducing production of amyloid β protein in an amount sufficient to form a progressive neural disease in the hippocampus or a peripheral portion of the cerebral cortex of the brain.
Dependencies are inferred from the claim text.