US 7,022,836 B2 · Methods for producing and transforming cassava protoplasts

Abstract

The invention relates to a method for producing protoplasts of cassava or closely related species, which protoplasts are capable of regeneration into plants. The method comprises producing friable embryogenic callus from explants of cassava or closely related species and isolating protoplasts from said friable embryogenic callus. The invention also concerns protoplasts obtainable by said method. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. In addition, the invention concerns a method for regenerating plants from these protoplasts and a cassava plant or closely related species obtainable thereby. Starch isolated from tubers of such cassava plants have an increased amylopectin content. The starch and a method for isolating it from said plants also forms part of the invention.

Claims10

Dependent claims

A starch obtainable by isolating starch from a tuber of a cassava plant obtainable by regenerating plants from protoplasts wherein the protoplast is induced to produce an embryo, which embryo is consequently induced to produce a plant;

wherein the protoplast is produced by the method which comprises producing friable embryogenic callus from explants of cassava and isolating protoplasts from said friable embryogenic callus;
wherein the isolation comprises the steps of:
washing the tuber, followed by grating and milling it;
separating starch from fibers and juice in a separator;
sieving the starch;
washing the starch;
and
drying the starch,
said starch having an amylopectin content of at least 95 wt. % based on the (dry substance) weight of the starch.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/119,226Filed 9 Apr 2002 · continuation in part

Priority

1 Feb 1999

Published

US 2003/0124724 A13 Jul 2003

Granted

4 Apr 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12N 15/8207
By physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated: by mechanical means, e.g. microinjection, particle…
A01H 4/005
Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor: methods for micropropagation; Vegetative plant propagation…
A01H 4/008
Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor: methods for regeneration to complete plants
C08B 30/04
Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin: extraction or purification

Family

Application09/180,481Parent of this continuation-in-part · filed 1 Feb 1999

Application09/832,626Parent of this continuation-in-part · filed 11 Apr 2001

US 6,551,827 B1Parent of this continuation-in-part · filed 22 Apr 2003

US 2003/0124724 A1Pre-grant publication · published 3 Jul 2003

US 2002/0049997 A1Published application

US 6,982,327 B2Related patent

US 7,022,836 B2This patent

From the US filing history. Foreign family members aren’t listed.

Methods for producing and transforming cassava protoplasts

Life of this patent

Methods for producing and transforming cassava protoplasts

Life of this patent

Abstract

Claims10