Method for binding nucleic acids to a solid phase
AssigneeMax-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
InventorsHolger Rauth, Richard Reinhardt, Eckard Nordhoff
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Abstract
The invention concerns a method for binding nucleic acids to a solid phase in which a nucleic acid containing solution is contacted with a solid phase which has hydrophobic and hydrophilic groups on its surface or/and which comprises a hydrophilic, water-containing polymer matrix, in the presence of dehydrating reagents, in particular in the presence of a salt and polyethylene glycol whereby the nucleic acid is bound to the surface.
Claims27
A method for binding nucleic acids to a solid phase comprising contacting a solution containing nucleic acids with a solid phase containing hydrophobic groups for binding nucleic acids and hydrophilic groups for avoiding agglomeration of said solid phase on its surface, wherein a salt and polyethylene glycol are present in said solution during binding of the nucleic acids to said solid phase and said nucleic acids are reversibly and sequence-unspecifically bound to the surface via said hydrophobic groups.
A method for isolating or/and purifying nucleic acids comprising
- (a) providing a solution containing nucleic acids,
- (b) contacting the solution containing nucleic acids with a solid phase which has hydrophobic and hydrophilic groups on its surface, wherein a salt and polyethylene glycol are present in said solution during binding of the nucleic acids via said hydrophobic groups to said solid phase and the nucleic acid is reversibly and sequence-unspecifically bound to the surface of the solid phase, and
- (c) separating the solid phase from the solution.
A method for detecting an analyte in a sample, comprising
- contacting a solution containing nucleic acids with a solid phase, wherein said solid phase has hydrophobic and hydrophilic groups on the surface, and wherein a salt and polyethylene glycol are present in said solution during binding of the nucleic acids to said solid phase and the nucleic acids are reversibly and sequence-unspecifically bound to the surface of said solid phase via said hydrophobic groups,
- subsequently contacting the solid phase with a sample, and
- detecting any analyte by means of the binding to the bound nucleic acids.
Dependencies are inferred from the claim text.