MurC gene and enzyme of Pseudomonas aeruginosa
AssigneeMerck & Co., Inc.
InventorsMohamend El-Sherbeini, Barbara Azzolina
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Abstract
This invention provides isolated polynucleotides that encode the MurC protein of Pseudomonas aeruginosa. Purified and isolated MurC recombinant proteins are also provided. Nucleic acid sequences which encode functionally active MurC proteins are described. Assays for the identification of modulators of the of expression of murC and inhibitors of the activity of MurC, are also provided.
Claims11
A purified and isolated polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO:
- 2.
A purified and isolated polypeptide having the amino acid sequence of SEQ ID NO:
- 2.
A method of determining whether a candidate compound is an inhibitor of a Pseudomonas aeruginosa MurC polypeptide comprising:
- (a) providing at least one host cell harboring an expression vector that includes a polynucleotide encoding a polypeptide having the amino acid sequence of SEQ ID NO:
- 2, and
- (b) culturing said host cell under conditions that promote the expression of said polypeptide, and
- (c) contacting said at least one cell with the candidate to permit the interaction of the candidate with the MurC polypeptide, and
- (d) determining whether the candidate is an inhibitor of the MurC polypeptide by ascertaining the activity of the polypeptide in the presence of the candidate.
A method of determining whether a candidate compound is an inhibitor of a Pseudomonas aeruginosa MurC polypeptide comprising:
- (a) providing a sample that includes a MurC polypeptide having the amino acid sequence of SEQ ID NO:
- 2, and
- (b) contacting said sample with the candidate to permit the interaction of the candidate with the MurC polypeptide, and
- (c) determining whether the candidate is an inhibitor of the MurC polypeptide by ascertaining the activity of the MurC polypeptide in the presence of the candidate.
Dependencies are inferred from the claim text.