Xylanase, microorganisms producing it, DNA molecules, methods for preparing this xylanase and uses of the latter
AssigneeGenencor International, Inc.
InventorsEric De Buyl, Andrée Lahaye, Pierre Ledoux, René Detroz
Life of this patent
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Abstract
The invention relates to an isolated and purified culture of Bacillus sp. strain 720/1 (LMG P-14798); to xylanases obtained from this strain and xylanases obtained from derivatives and mutants of strain 720/1. The invention also relates to a DNA molecule encoding a xylanase and to expression vectors or integration vectors containing the DNA molecule. This invention also relates to transformed host strains comprising the DNA molecule encoding the xylanase.
Claims14
An isolated DNA molecule, wherein said DNA molecule comprises SEQ ID NO:
- 1.
An isolated promoter comprising the sequence illustrated in SEQ ID NO:
- 26, wherein said promoter is derived from the gene which codes for Bacillus pumilus PRL B12 xylanase.
An isolated presequence comprising the sequence illustrated in SEQ ID NO:
- 27 which codes for the signal peptide of Bacillus pumilus PRL B12 xylanase.
An expression system which can be used for the production of a polypeptide, comprising:
- a) a promoter comprising the sequence illustrated in SEQ ID NO:
- 26 derived from the gene which codes for Bacillus pumilus PRL B12 xylanase,
- b) a sequence coding for a signal peptide, and
- c) a sequence encoding the polypeptide.
An expression system which can be used for the production of a polypeptide comprising:
- a) a promoter;
- b) a presequence having the sequence illustrated in SEQ ID NO:
- 27 which codes for the signal peptide of Bacillus pumilus PRL B12 xylanase, and
- c) a sequence encoding the polypeptide.
An expression system which can be used for the production of a polypeptide comprising:
- a) a promoter having the sequence illustrated in SEQ ID NO:
- 26 derived from the gene which codes for Bacillus pumilus PRL B12 xylanase;
- b) a presequence having the sequence illustrated in SEQ ID NO:
- 27 which codes for the signal peptide of Bacillus pumilus PRL B12 xylanase,
- c) a sequence encoding the polypeptide, and
- d) a terminator sequence.
Dependencies are inferred from the claim text.