Composition for inoculating legumes and method therefor
OwnerJuan Bautista Mario Lucio Magriinventor
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Abstract
A method for preparing a concentrated inoculating composition which contains a universal use culture medium which is sterilized and cooled, then added to Rhizobium Japonicum and then allowed to multiply for 46 hours. This is followed by the addition to the culture medium of powdered maltose, liquid maltose and lactose saccharides as well as the fungicide potassium sorbate. The general use culture medium can comprise previously treated peat as one of its components. The above results in an inoculating composition for use with leguminous plants with or without peat incorporated as part of the general use culture medium.
Claims2
A method for preparing a concentrated inoculating composition for use with leguminous plants, comprising me following steps in the order given:
- a) sterilize and cool a culture medium suitable for supporting the growth of Rhizobium japonicum bacteria;
- b) add Rhizobium japonicum bacteria to the culture medium and allow to multiply for 46 hours at a temperature of 28 C.;
- c) cool the result of step b) to a temperature of 10° C. or 11° C.;
- d) add a solution comprising powdered maltose saccharide, liquid maltose saccharide, lactose saccharide, and potassium sorbate to the combined culture medium and bacteria, said solution being prepared by the following steps:
- 1) dissolving said powdered maltose saccharide, said liquid maltose saccharide, and said potassium sorbate in 100 liters of water;
- 2) steriling at 121° C. for 30 minutes;
- 3) cooling to 20° C.
Dependencies are inferred from the claim text.