Reducing branching and enhancing fragmentation in culturing filamentous microorganisms
AssigneeRijksuniversiteit te Leiden
InventorsGilles Philippus van Wezel, Barend Kraal, Rudolf Gijsbertus M. Luiten
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Abstract
The invention relates to the field of microorganisms and to the culturing of microorganisms. Means and methods are described for enhancing the culturing properties of filamentous microorganisms, particularly filamentous fungi. According to the invention, the means and methods generally comprise reducing the branching and/or enhancing the fragmentation of the microorganisms, so that their liquid culturing properties are improved. In one embodiment, this is achieved by providing the microorganisms with activity capable of enhancing fragmentation and/or reducing branching such as the activity which in, for example, Streptomyces griseus is encoded by ssgA.
Claims13
A method for producing a recombinant Streptomyces bacterium, said method comprising:
- transforming or transfecting a Streptomyces bacterium with an expressible polynucleotide encoding a heterologous SsgA that is not present in the Streptomyces bacterium in nature, the heterologous SsgA comprising at least one of SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7 and SEQ ID NO:
- 9, said Streptomyces bacterium lacking detectable endogenous SsgA during submerged culture.
A method for producing a recombinant Actinomycete bacterium, said method comprising:
- transforming an Actinomycete bacterium lacking a detectable endogenous SsgA with a nucleic acid encoding a heterologous SsgA comprising at least one of SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7 and SEQ ID NO:
- 9:
- wherein the Actinomycete bacterium is selected from the group consisting of Streptomyces clavuligerus and Streptomyces eryhraea.
A method for producing a recombinant Saccharopolyspora bacterium, said method comprising transforming a Saccharopolyspora bacterium with an expressible polynucleotide encoding a heterologous SsgA comprising at least one of SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7 and SEQ ID NO:
- 9.
Dependencies are inferred from the claim text.