Cell culture media for mammalian cells

A composition comprising a cell culture medium and pancreatic islet cells cultured in said cell culture medium in vitro, said culture medium comprising 1–150 mg/L arginine;

• 1–120 mg/L proline;
• 1–3050 mg/L nicotinamide;
• 0.1–100 mg/L transferrin chelated with iron;
• greater than 10−11 M insulin or insulin-like growth factors;
• 10−12 M-103 M glucocorticoid steroid;
• 1–6000 μg/L zinc salt;
• 1–250 μg/L manganese salt;
• 1–1000 μg/L copper salt;
• 1–150 μg/L selenium salt;
• 2.0–10.0 mM L-glutamine;
• 0.01–5.0 g/L D-galactose or 0.01–5.0 g/L D-glucose, or when both D-galactose and D-glucose are included together, 0.01–8.0 g/L.