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US 7,022,493 B2Granted
Ubiquitin conjugation assays
AssigneeRigel Pharmaceuticals, Inc.
InventorsSarkiz D. Issakani, Jianing Huang, Julie Sheung, Todd R. Pray
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Abstract
The invention relates to assays for measuring ubiquitin ligase activity and for identifying modulators of ubiquitin ligase enzymes.
Claims20
A method of identifying a ubiquitination modulator comprising:
- a) combining, under conditions that favor ubiquitination activity:
- i) tag1-ubiquitin;
- ii) a candidate modulator;
- iii) ubiquitin activating enzyme (E1);
- and
- iv) tag2-ubiquitin conjugating enzyme (E2);
- b) measuring the amount of tag1-ubiquitin bound to said tag2-ubiquitin conjugating enzyme (E2), whereby a difference in bound ubiquitin as compared with a reaction performed in the absence of the candidate modulator indicates that the candidate is a ubiquitination modulator.
4 more elements
Dependencies are inferred from the claim text.