Method for determining molecule—molecule interaction analysis
OwnerPeter Jamesinventor
Life of this patent
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Abstract
The invention relates to a method for monitoring interactions to a target biomolecule comprising the steps of: providing a biomolecule of interest having specificity for the target biomolecule; binding the biomolecule of interest to at least one type of linker molecule comprising a unique mass marker part; introducing the biomolecule of interest to a cell; binding the linker to the target biomolecule; cleaving the linker molecule, thereby leaving the photoactivatable part and the mass marker part bound to the target; analysing the target biomolecule, thereby detecting the unique mass marker part. The detection can be carried out by MS in a parent ion scanning mode, thereby allowing study of the interaction between the biomolecule of interest and the target biomolecule.
Claims25
A method for identifying an interacting target biomolecule to a biomolecule of interest comprising the steps of:
- (a) providing a biomolecule of interest having specificity for the target;
- (b) binding the biomolecule of interest to at least one type of linker molecule, the linker molecule including at least one attachment part for binding to the biomolecule of interest, one cleavable part, one mass marker part and one photoactivatable part, for binding to the target;
- (c) contacting the biomolecule of interest with a cell or a cell extract;
- (d) exposing the cell to photolysis, whereby the photoactivatable part binds to the target;
- (e) cleaving the linker molecule or molecules, thereby leaving the photoactivatable part and the mass marker part bound to the target;
- and
- (f) analysing the product of step (e), thereby detecting the mass marker part, thus identifying the interacting target biomolecule to the biomolecule of interest.
Dependencies are inferred from the claim text.