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US 7,022,490 B1Granted
Myc-binding zinc finger proteins, their preparation and their use
AssigneeProlifix Limited
InventorsKaren Peukert, Frank Haenel, Martin Eilers
Life of this patent
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Abstract
The present invention provides assays for determining transcription-modulating substances. The transcription-modulating activity of test compounds is assessed on the basis of their ability to suppress or enhance complex formation between Myc and a novel zinc finger binding protein, Miz-1, or derivatives thereof.
Claims8
A method for identifying specific transcription-modulating substances which comprises the following steps
- (a) incubating a protein, the protein having the amino acid sequence depicted in SEQ ID NO:2 or being a mutein thereof obtainable by substitution, deletion or insertion of 1 to 100 amino acids and retaining the ability to bind specifically to myc, with the myc gene product under conditions such that a complex between these two proteins is formed,
- (b) incubating the two proteins in the presence of one or more substances which are to be tested for specific transcription-modulating activities but under conditions which are otherwise the same as in (a),
- (c) determining the difference in the protein complex formation between (b) and (a),
- (d) selecting those substances with which the protein complex formation in step (b) is different from that in step (a).
1 more element
A method for identifying specific transcription-modulating substances which comprises the following steps
- (a) incubating a protein, the protein comprising the amino acid sequence of SEQ ID NO:2 or a fragment thereof retaining the ability to bind specifically to myc, with the myc gene product under conditions such that a complex between these two proteins is formed,
- (b) incubating the two proteins in the presence of one or more substances which are to be tested for specific transcription-modulating activities but under conditions which are otherwise the same as in (a),
- (c) determining the difference in the protein complex formation between (b) and (a),
- (d) selecting those substances with which the protein complex formation in step (b) is different from that in step (a).
1 more element
Dependencies are inferred from the claim text.