Method of using cells expressing galanin receptor 3 (GALR3)

A process for preparing a composition which comprises identifying a chemical compound which specifically binds to a human or rat GALR3 receptor, recovering the chemical compound free of any receptor, and then admixing a carrier and the chemical compound, wherein the chemical compound is identified by a process involving competitive binding which comprises:

• (a) contacting cells transfected with DNA or injected with RNA encoding and expressing on their cell surface the human or rat GALR3 receptor or a membrane fraction from such cells, with both the chemical compound and a second chemical compound known to bind to the receptor, and separately with only the second chemical compound, under conditions suitable for binding of both compounds, and
• (b) detecting specific binding of the chemical compound to the human or rat GALR3 receptor, a decrease in the binding of the second chemical compound to the human or rat GALR3 receptor in the presence of the chemical compound indicating that the chemical compound binds to the human or rat GALR3 receptor;
• wherein such cells prior to being transfected with such DNA do not express the human or rat GALR3 receptor, and wherein the human GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 4 (SEQ ID NO:
• 4) or that encoded by plasmid pEXJ-hGalR3 (ATCC Accession No. 97827);
• and the rat GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 2 (SEQ ID NO:
• 2) or that encoded by plasmid pEXJ-RGalR3T (ATCC Accession No. 97826) or plasmid K1086 (ATCC Accession No. 97747).

A process for preparing a composition which comprises determining whether a chemical compound is a GALR3 receptor agonist, recovering the chemical compound free of any receptor, and then admixing a carrier and the chemical compound wherein the chemical compound is determined as a GALR3 receptor agonist by a process which comprises:

• (a) contacting cells transfected with DNA or injected with RNA encoding and expressing on their cell surface the human or rat GALR3 receptor or a membrane fraction from such cells, with the compound under conditions permitting the activation of the GALR3 receptor, and
• (b) detecting an increase in GALR3 receptor activity, so as to thereby determine whether the compound is a GALR3 receptor agonist;
• wherein such cells prior to being transfected with such DNA do not express the human or rat GALR3 receptor, and wherein the human GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 4 (SEQ ID NO:
• 4) or that encoded by plasmid pEXJ-hGalR3 (ATCC Accession No. 97827);
• and the rat GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 2 (SEQ ID NO:
• 2) or that encoded by plasmid pEXJ-RGalR3T (ATCC Accession No. 97826) or plasmid K1086 (ATCC Accession No. 97747).

A process for preparing a composition which comprises determining whether a chemical compound is a GALR3 receptor agonist, recovering the chemical compound free of any receptor, and then admixing a carrier and the chemical compound wherein the chemical compound is determined as a GALR3 receptor agonist by a process which comprises:

• (a) contacting cells transfected with DNA or injected with RNA encoding and expressing on their cell surface the human or rat GALR3 receptor or a membrane fraction from such cells, with the compound in the presence of a known GALR3 receptor agonist, under conditions permitting the activation of the GALR3 receptor, and
• (b) detecting a decrease in GALR3 receptor activity, so as to thereby determine whether the compound is a GALR3 receptor agonist;
• wherein such cells prior to being transfected with such DNA do not express the human or rat GALR3 receptor, and wherein the human GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 4 (SEQ ID NO:
• 4) or that encoded by plasmid pEXJ-hGalR3 (ATCC Accession No. 97827);
• and the rat GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 2 (SEQ ID NO:
• 2) or that encoded by plasmid pEXJ-RGalR3T (ATCC Accession No. 97826) or plasmid K1086 (ATCC Accession No. 97747).

A process for preparing a composition which comprises determining whether a chemical compound specifically binds to and activates a human or a rat GALR3 receptor, recovering the chemical compound free of any receptor, and the admixing a carrier and the chemical compound, wherein the chemical compound is determined to bind to and activate a human or rat GALR3 receptor by a process which comprises:

• (a) contacting cells producing a second messenger response and transfected with DNA or injected with RNA encoding and expressing on their cell surface the human or rat GALR3 receptor, wherein such cells prior to being transfected with such DNA do not express the human or rat GALR3 receptor, with the chemical compound under conditions suitable for activation of the human or the rat GALR3 receptor, and
• (b) measuring the second messenger response in the presence and in the absence of the chemical compound, a change in second messenger response in the presence of the chemical compound indicating that the chemical compound activates the human or the rat GALR3 receptor;
• wherein the human GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 4 (SEQ ID NO:
• 4) or that encoded by plasmid pEXJ-hGalR3 (ATCC Accession No. 97827);
• and the rat GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 2 (SEQ ID NO:
• 2) or that encoded by plasmid pEXJ-RGalR3T (ATCC Accession No. 97826) or plasmid K1086 (ATCC Accession No. 97747).

A process for preparing a composition which comprises determining whether a chemical compound specifically binds to and inhibits activation of a human or rat GALR3 receptor, recovering the chemical compound free of any receptor, and then admixing a carrier and the chemical compound, wherein the chemical compound is determined to bind to and inhibit activation of a human or rat GALR3 receptor by a process which comprises:

• (a) separately contacting cells producing a second messenger response and transfected with DNA or injected with RNA encoding and expressing on their cell surface the human or the rat GALR3 receptor, wherein such cells prior to being transfected with such DNA do not express the human or rat GALR3 receptor, with both the chemical compound and a second chemical compound known to activate the human or rat GALR3 receptor, and with only the second chemical compound, under conditions suitable for activation of the GALR3 receptor, and
• (b) measuring the second messenger response in the presence of oily the second chemical compound and in the presence of both the second chemical compound and the chemical compound,
• (c) a smaller change in the second messenger response in the presence of both the chemical compound and the second chemical compound than in the presence of only the second chemical compound indicating that the chemical compound inhibits activation of the human or rat GALR3 receptor;
• wherein the human GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 4 (SEQ ID NO:
• 4) or that encoded by plasmid pEXJ-hGalR3 (ATCC Accession No. 97827);
• and the rat GALR3 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 2 (SEQ ID NO:
• 2) or that encoded by plasmid pEXJ-RGalR3T (ATCC Accession No. 97826) or plasmid K1086 (ATCC Accession No. 97747).