Detection of target substances utilizing biochemically specific binding reaction
AssigneeFuji Photo Film Co., Ltd.
InventorKeiko Neriishi
Life of this patent
Estimating the term…
Abstract
A composite material sheet which is composed of partitions two-dimensionally extending on a sheet plane to form on the sheet plane plural fine sections surrounded by the partitions and porous material portions each of which is placed in the fine section, or a simple porous sheet is employed for autoradiographic analysis of substances originating from living body or its analogues in combination with a stimulable phosphor sheet which is composed of a support and fine stimulable phosphor layers distributed on the support in such manner that each stimulable phosphor layer takes a position corresponding to each fine section of the composite material sheet or a position of the porous sheet in which probe molecules are spotted.
Claims11
A process for detecting complementary nucleic acid fragments which comprises the steps of:
- preparing a composite material sheet, wherein said composite material sheet comprises partitions two-dimensionally extending on a sheet plane to form 10 to 10,000 per cm2 fine sections on the sheet plane, and a porous material portion placed in each fine section, wherein a group of single-stranded probe nucleic acid fragments are attached to each porous material portion;
- bringing radioactively labeled single-stranded sample nucleic acid fragments in a liquid phase, into contact with the prepared composite material sheet, whereby single-stranded target nucleic acid fragments amoung the sample nucleic acid fragments are fixed by hybridization to probe nucleic acid fragments;
- removing unfixed sample nucleic acid fragments from the composite material sheet;
- placing the composite material sheet having target nucleic acid fragments fixed by hybridization in contact with a stimulable phosphor sheet, wherein the stimulable phosphor sheet comprises a support and fine stimulable phosphor layers distributed on the support in such manner that each stimulable phosphor layer takes a position corresponding to each fine section of the composite material sheet, whereby the stimulable phosphor layers absorb and store radiation energy of the radioactive label coming from the fixed nucleic acid fragments;
- irradiating the stimulable phosphor sheet with a stimulating light, whereby the stimulable phosphor layers storing the radiation energy release stimulated emissions;
- detecting the stimulated emissions photoelectrically to obtain a series of electric signals;
- and
- processing the electric signals to detect areas in which the target nucleic acid fragments are fixed.
A process for detecting complementary nucleic acid fragments which comprises the steps of:
- preparing a composite material sheet, wherein said composite material sheet comprises partitions two-dimensionally extending on a sheet plane to form plural fine sections, on the sheet plane, having an opening with a mean area in the range of 0.001 to 5 mm2, and a porous material portion placed in each fine section, wherein a group of single-stranded probe nucleic acid fragments are attached to each porous material portion;
- bringing radioactively labeled single-stranded sample nucleic acid fragments in a liquid phase, into contact with the prepared composite material sheet, whereby single-stranded target nucleic acid fragments among the sample nucleic acid fragments are fixed by hybridization to probe nucleic acid fragments;
- removing unfixed sample nucleic acid fragments from the composite material sheet;
- placing the composite material sheet having target nucleic acid fragments fixed by hybridization in contact with a stimulable phosphor sheet, wherein said stimulable phosphor sheet comprises a support and fine stimulable phosphor layers distributed on the support in such manner that each stimulable phosphor layer takes a position corresponding to each fine section of the composite material sheet, whereby the stimulable phosphor layers absorb and store radiation energy of the radioactive label coming from the fixed nucleic acid fragments;
- irradiating the stimulable phosphor sheet with a stimulating light, whereby the stimulable phosphor layers storing the radiation energy release stimulated emissions;
- detecting the stimulated emissions photoelectrically to obtain a series of electric signals;
- and
- processing the electric signals to detect areas in which the target nucleic acid fragments are fixed.
Dependencies are inferred from the claim text.