Phytase enzymes, nucleic acids encoding phytase enzymes and vectors and host cells incorporating same
AssigneesGenencor International, Inc., The University of Manchester
InventorsChristian F. Stafford, Anthony P. J. Trinci, Jayne L. Brookman
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Abstract
A novel DNA is provided which encodes an enzyme having phytase activity isolated from Penicillium. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.
Claims10
An isolated phytase derived from a Penicillium species, said phytase being encoded by a nucleotide sequence capable of hybridizing to a polynucleotide sequence as shown in SEQ ID NO. 1, SEQ ID NO. 2, or SEQ ID NO:
- 3 under conditions of high stringency said conditions occurring about 5 to 10° C. below the melting temperature of the sequence shown in SEQ ID NO:
- 1, SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- and said phytase having one or more of the following physiochemical properties:
- (1) molecular weight:
- between about 45–60 kDa (non-glycosylated);
- (2) a theoretical pl of between about 7 and 7.6;
- (3) a pH optimum within a range of about 4.5–5.5 or
- (4) an ambient temperature optimum with a range of from about 40 to about 45 degree C.
An isolated phytase enzyme having at least 95% amino acid sequence identity to SEQ ID NO:
- 4.
An isolated phytase enzyme comprising the amino acid sequence disclosed in SEQ 10 NO:
- 4.
A food or animal feed including an enzyme having phytase activity, wherein said enzyme comprises an amino acid sequence having at least 95% identity to an amino add sequence as disclosed in SEQ ID NO:
- 4.
A food or animal feed including an enzyme having phytase activity, wherein said enzyme comprises an amino add sequence having at least 95% identity to an amino add sequence as disclosed in SEQ ID NO:
- 4, wherein said enzyme is derived from a Penicillium hordei.
Dependencies are inferred from the claim text.