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US 7,022,354 B1Granted
Process for producing fermentation product
AssigneeSapporo Breweries Limited
InventorsMasahide Sato, Shingo Umemoto
Life of this patent
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Abstract
A process for producing a fermentation product by effecting fermentation with the use of a bioreactor having an immobilized microorganism located therein, characterized in that a non-flocculent yeast is employed as the microorganism.
Claims30
A method for making a fermentation product comprising:
- immobilizing a non-flocculent yeast in a bioreactor,
- contacting a fermentation liquid with the immobilized non-flocculent yeast for a time and under conditions suitable for fermentation, and
- recovering the fermentation product(s),
- wherein said non-flocculent yeast is immobilized on a carrier selected from the group consisting of a chitosan bead, alginic acid and carrageenan and
- wherein said non-flocculent yeast satisfies the following:
- a suspension of 0.6 g of said yeast is mixed with 20 ml of water,
- 1 ml of 0.5 M acetic acid buffer solution at pH 4.5 including 1,500 ppm calcium ion is added to 9 ml of said suspension,
- the resulting mixture is left to stand still for 5 minutes at room temperature, and
- neither flocculation nor sedimentation of said yeast is observed.
6 more elements
A fermentation method comprising:
- immobilizing a non-flocculent yeast on an immobilizing carrier within a bioreactor having a fluidized bed section,
- supplying the bioreactor with a fermentation liquid,
- extracting a part of the fermentation liquid from the downstream side of the fluidized bed section and returning the part of the fermentation liquid to the upstream side of the fluidized bed section, while forming a fluidized bed to carry out the fermentation of the fermentation liquid;
- and
- recovering the thus obtained fermentation product from the bioreactor and
- optionally supplying the bioreactor with a new fermentation liquid to repeat the fermentation,
- wherein said non-flocculent yeast satisfies the following:
- a suspension of 0.6 g of said yeast is mixed with 20 ml of water,
- 1 ml of 0.5 M acetic acid buffer solution at pH 4.5 including 1,500 ppm calcium ion is added to 9 ml of said suspension,
- the resulting mixture is left to stand still for 5 minutes at room temperature, and
- neither flocculation nor sedimentation of said yeast is observed.
8 more elements
A method for making a fermentation product comprising:
- immobilizing a non-flocculent yeast in a fluidized bed bioreactor on the surface of a chitosan bead,
- contacting a fermentation liquid with the immobilized non-flocculent yeast for a time and under conditions suitable for fermentation,
- recovering the primary fermentation product(s),
- wherein the primary fermentation product(s) have a higher number of floating yeast cells that the primary fermentation product(s) obtained using a similar flocculent yeast of the same species, and
- wherein said non-flocculent yeast satisfies the following:
- a suspension of 0.6 g of said yeast is mixed with 20 ml of water,
- 1 ml of 0.5 M acetic acid buffer solution at pH 4.5 including 1,500 ppm calcium ion is added to 9 ml of said suspension,
- the resulting mixture is left to stand still for 5 minutes at room temperature, and
- neither flocculation nor sedimentation of said yeast is observed.
6 more elements
Dependencies are inferred from the claim text.